Department of Biological Sciences, University of Maryland, Baltimore County, 21228, Catonsville, MD, USA.
Theor Appl Genet. 1984 Jan;67(2-3):185-93. doi: 10.1007/BF00317030.
A physical map containing six restriction sites of the Nicotiana tabacum chloroplast genome, together with the BamHI maps of N. tabacum, N. otophora and N. knightiana, and the SmaI maps of N. acuminata, N. plumbaginifolia, N. langsdorffii, N. otophora, N. tabacum, N. tomentosiformis and N. knightiana was constructed. In Nicotiana chloroplast genomes, the most frequently observed variations are point mutations. Deletions are also detected. Most of the observed changes are confined to one area of the large single copy region, which is designated as the "hot spot". Based on the evidence obtained from Nicotiana chloroplast genomes, an origin of the inverted repeats in this genus is proposed. We suggest that the inverted repeats represent a vestige of what were once two identical, complete chloroplast genomes joined together in a head-to-head and tail-to-tail fashion, and that deletions generated the current chloroplast genome organization.
构建了一个包含烟草叶绿体基因组 6 个限制位点的物理图谱，以及烟草、黄花烟草、肾叶烟草的 BamHI 图谱，以及黄花烟草、菸草、少花烟草、黄花烟草、烟草、毛状烟草和肾叶烟草的 SmaI 图谱。在烟草叶绿体基因组中，最常见的变异是点突变。也检测到缺失。大多数观察到的变化仅限于大单一拷贝区域的一个区域，该区域被指定为“热点”。基于从烟草叶绿体基因组获得的证据，提出了该属中反向重复的起源。我们认为，反向重复代表了曾经是两个完全相同的完整叶绿体基因组以头对头和尾对尾的方式连接在一起的遗迹，而缺失产生了当前的叶绿体基因组结构。
