甜菜（Beta vulgaris L.）叶绿体 DNA 的限制酶切图谱。

Restriction fragment map of sugar beet (Beta vulgaris L.) chloroplast DNA.

机构信息

Plant Breeding Institute, Faculty of Agriculture, Hokkaido University, 060, Sapporo, Japan.

出版信息

Plant Mol Biol. 1986 May;7(3):201-5. doi: 10.1007/BF00021331.

Abstract

A restriction endonuclease fragment map of sugar beet chloroplast DNA (ctDNA) has been constructed with the enzymes SmaI, PstI and PvuII. The ctDNA was found to be contained in a circular molecule of 148.5 kbp. In common with many other higher plant ctDNAs, sugar beet ctDNA consists of two inverted repeat sequences of about 20.5 kbp separated by two single-copy regions of different sizes (about 23.2 and 84.3 kbp). Southern hybridization analyses indicated that the genes for rRNAs (23S+16S) and the large subunit of ribulose 1,5-bisphosphate carboxylase were located in the inverted repeats and the large single-copy regions, respectively.

摘要

已构建了甜菜叶绿体 DNA (ctDNA) 的 SmaI、PstI 和 PvuII 限制内切酶片段图谱。ctDNA 被发现包含在一个 148.5 kbp 的环状分子中。与许多其他高等植物 ctDNA 一样，甜菜 ctDNA 由大约 20.5 kbp 的两个反向重复序列组成，它们被两个大小不同的单拷贝区隔开（约 23.2 和 84.3 kbp）。Southern 杂交分析表明，rRNA（23S+16S）和核酮糖 1,5-二磷酸羧化酶大亚基的基因分别位于反向重复序列和大单拷贝区。