Shinozaki K, Sugiura M
Gene. 1982 Nov;20(1):91-102. doi: 10.1016/0378-1119(82)90090-7.
The gene for the large subunit (LS) of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCase/Oase) from tobacco has been cloned in pBR322 and sequenced. The coding region contains 1431 bp (477 codons). The deduced amino acid sequence of tobacco LS protein shows 90% homology with those of maize and spinach LS. The positions in the gene corresponding to the 5' and the 3' ends of tobacco LS mRNA have been located on the DNA sequence by the S1 nuclease mapping procedure. The LS gene promoter sequence has homology with Escherichia coli promoter sequences; its terminator sequence is capable of forming a stem-and-loop structure. A sequence GGAGG, which is complementary to a sequence near the 3' end of tobacco chloroplast 16S rRNA and a putative ribosome binding site, occurs 6-10 bp upstream from the initiation codon.
已将来自烟草的1,5 - 二磷酸核酮糖羧化酶/加氧酶（RuBPCase/Oase）大亚基（LS）的基因克隆到pBR322中并进行了测序。编码区包含1431个碱基对（477个密码子）。推导的烟草LS蛋白氨基酸序列与玉米和菠菜的LS序列显示出90%的同源性。通过S1核酸酶图谱分析程序，已在DNA序列上定位了烟草LS mRNA 5'和3'末端在基因中对应的位置。LS基因启动子序列与大肠杆菌启动子序列具有同源性；其终止子序列能够形成茎环结构。一个与烟草叶绿体16S rRNA 3'末端附近序列互补的序列GGAGG以及一个假定的核糖体结合位点，出现在起始密码子上游6 - 10个碱基对处。
