Centre of Agrobiological Research, Postbox 14, NL-6700 AA, Wageningen, The Netherlands.
Plant Cell Rep. 1981 Aug;1(1):18-20. doi: 10.1007/BF00267649.
Plasmodia have been isolated from Brassica campestris tissue infected with Plasmodiophora brassicae. Cytokinins were extracted from plasmodia and from the remaining host cytoplasm. Fractions were separated in a butanol soluble fraction containing free cytokinins and a water soluble fraction containing bound forms, presumably glucose-6-phosphate derivatives of zeatin and zeatin riboside. The butanol fraction was analysed by high pressure liquid chromatography (HPLC). The different glucosides were determined after treatment of the water fraction with β-glucosidase.Plasmodia contained zeatin riboside and its glucose derivative (175 and 1600 ng/g dry weight, respectively). Host cytoplasm contained zeatin riboside (132 ng/g dry weight) and small amounts of the glucose-6-phosphate derivatives of zeatin and zeatin riboside. The results suggest that plasmodia synthesize cytokinins which are released into the host cytoplasm inducing host cell division.
已从受根肿菌浸染的芸薹属植物组织中分离出疟原虫。从疟原虫和剩余的宿主细胞质中提取细胞分裂素。在丁醇可溶部分（含游离细胞分裂素）和水溶部分（含结合形式，可能是玉米素和玉米素核苷的葡萄糖-6-磷酸衍生物）中分离出各个馏分。丁醇馏分用高压液相色谱法（HPLC）进行分析。水馏分经β-葡萄糖苷酶处理后，测定不同的葡萄糖苷。疟原虫中含有玉米素核苷及其葡萄糖衍生物（分别为 175 和 1600ng/g 干重）。宿主细胞质中含有玉米素核苷（132ng/g 干重）和少量玉米素和玉米素核苷的葡萄糖-6-磷酸衍生物。这些结果表明，疟原虫合成细胞分裂素，然后将其释放到宿主细胞质中，诱导宿主细胞分裂。
