Department of Horticulture, Sunchon National University, Suncheon 57922, Korea.
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
Int J Mol Sci. 2020 May 29;21(11):3896. doi: 10.3390/ijms21113896.
The obligate biotroph causes clubroot disease in oilseeds and vegetables of the Brassicaceae family, and cytokinins play a vital role in clubroot formation. In this study, we examined the expression patterns of 17 cytokinin-related genes involved in the biosynthesis, signaling, and degradation in Chinese cabbage inoculated with the Korean pathotype group 4 isolate of , Seosan. This isolate produced the most severe clubroot symptoms in Chinese cabbage cultivar "Bullam-3-ho" compared to three other Korean geographical isolates investigated. , a cytokinin biosynthesis gene, was induced on Day 1 and Day 28 in infected root tissues and the upregulation of this biosynthetic gene coincided with the higher expression of the response regulators , on both Days and on Day 1 and 3. and genes were also induced during gall enlargement on Day 35 in leaf tissues. The gene, which positively interact with phytochrome B, was consistently induced in leaf tissues on Day 1, 3, and 14 in the inoculated plants. The cytokinin degrading gene were induced on Day 14, before gall initiation. were induced until Day 28 and their expression was downregulated on Day 35. This insight improves our current understanding of the role of cytokinin signaling genes in clubroot disease development.
专性活体营养型生物引起十字花科油料作物和蔬菜的根肿病，细胞分裂素在根肿病的形成中起着至关重要的作用。在这项研究中，我们研究了接种来自韩国 4 组分离株的根肿病后白菜中 17 个与细胞分裂素生物合成、信号转导和降解相关的基因的表达模式。与其他三种韩国地理分离株相比，该分离株在白菜品种“Bullam-3-ho”中引起的根肿病症状最为严重。细胞分裂素生物合成基因 在感染的根部组织中于第 1 天和第 28 天被诱导，该生物合成基因的上调与反应调节因子 的更高表达相吻合，在第 1 天和第 3 天均上调。 在叶片组织中，在第 35 天的肿瘤增大期间也被诱导。与phytochrome B 有正向相互作用的 基因在接种植株的叶片组织中于第 1、3 和 14 天持续诱导。细胞分裂素降解基因 于第 14 天，在肿瘤起始之前被诱导。 在第 14 天到第 28 天之间被诱导，其表达在第 35 天被下调。这一见解提高了我们对细胞分裂素信号转导基因在根肿病发展中的作用的理解。
