Chemical constituents of cultured cells of Euphorbia tirucalli and E. millii.

We induced calluses from two Euphorbia species and analyzed the lipids and pigments of their cells. Growth was promoted when malt extract was added to the medium for callus induction. The lipid constituents of both E. tirucalli and E. millii calluses were the same; sitosterol, stigmasterol, campesterol, palmitic acid and linoleic acid. In addition, an anthocyanin, cyanidin glycoside, was isolated from callus that had been induced from E. millii leaves cultured on medium containing 0.1 ppm 2,4-D.