Plant Germ-plasm Institute, Faculty of Agriculture, Kyoto University, Mozume, Muko, 617, Kyoto, Japan.
Theor Appl Genet. 1984 Apr;67(6):529-33. doi: 10.1007/BF00264899.
The esterase isozymes of 432 strains of foxtail millet, Setaria italica (L.) P. Beauv., collected from different areas throughout Eurasia, were investigated by gel isoelectric focusing. Five phenotypes were recognized, based on the combination of five major activity bands. Cross experiments among different phenotypes revealed these isozymes to be controlled by two codominant alleles and a null allele on the locus, Est-1, and three codominant alleles on another independent locus, Est-2. On locus Est-1, 388 strains had Est-1 (a), 41 had Est-1 (b) and three had Est-1 (null) alleles. Est-1 (a) was widely distributed throughout Eurasia, while the distribution of Est-1 (b) and Est-1 (null) was distinctly restricted. On locus Est-2, 417 strains had Est-2 (a), nine had Est-2 (b) and six had Est-2 (c) alleles. Est-2 (a) was widely distributed throughout Asia to Czechoslovakia, but was not detected in the western part of Europe. Est-2 (b) was found in all of the strains from the western part of Europe and in one of the Indian strains. Est-2 (c) was rarely found in Japan and China. The distribution of Est-2 (a) and -2 (b) might indicate some degree of phylogenetic differentiation between the Asian and the European strains. Polymorphism in both loci was observed only in Chinese strains.
采用凝胶等电聚焦技术对来自欧亚大陆不同地区的 432 株谷子（Setaria italica (L.) P. Beauv.）酯酶同工酶进行了研究。根据 5 条主要活性带的组合，识别出 5 种表型。不同表型之间的交叉实验表明，这些同工酶受位于 Est-1 基因座的两个共显性等位基因和一个无效等位基因以及另一个独立的 Est-2 基因座上的三个共显性等位基因控制。在 Est-1 基因座上，388 株具有 Est-1 (a)等位基因，41 株具有 Est-1 (b)等位基因，3 株具有 Est-1 (null)等位基因。Est-1 (a)广泛分布于欧亚大陆，而 Est-1 (b)和 Est-1 (null)的分布则明显受限。在 Est-2 基因座上，417 株具有 Est-2 (a)等位基因，9 株具有 Est-2 (b)等位基因，6 株具有 Est-2 (c)等位基因。Est-2 (a)广泛分布于亚洲至捷克斯洛伐克，但在欧洲西部未检测到。Est-2 (b)存在于欧洲西部的所有菌株和印度的一个菌株中。Est-2 (c)在日本和中国很少见。Est-2 (a)和 -2 (b)的分布可能表明亚洲和欧洲菌株之间存在一定程度的系统发育分化。两个基因座的多态性仅在中国菌株中观察到。
