Institute of Zoology and Botany, Academy of Sciences of the Estonian SSR, 21 Vanemuise St., 202400, Tartu, Estonian SSR (USSR).
Theor Appl Genet. 1984 Apr;67(6):535-40. doi: 10.1007/BF00264900.
Evolutionary electrophoretic variation of a NAD-specific aromatic alcohol dehydrogenase, AADH-E, in wheat and goatgrass species is described and discussed in comparison with a NAD-specific alcohol dehydrogenase (ADH-A) and a NADP-dependent AADH-B studied previously. Cultivated tetraploid emmer wheats (T. turgidum s. l.) and hexaploid bread wheats (T. aestivum s. l.) are all fixed for a heterozygous triplet, E(0.58)/E(0.64). The slowest isoenzyme, E(0.58), is controlled by a homoeoallelic gene on the chromosome arm 6AL of T. aestivum cv. 'Chinese Spring' and is inherent in all diploid wheats, T. monococcum s. Str., T. boeoticum s. l. and T. urartu. The fastest isoenzyme, E(0.64), is presumably controlled by the B- and D-genome homoeoalleles of the bread wheat and is the commonest alloenzyme of diploid goat-grasses, including Ae. speltaides and Ae. tauschii. The tetraploid T. timopheevii s. str. has a particular heterozygous triplet E(0.56)/E(0.71), whereas the hexaploid T. zhukovskyi exhibited polymorphism with electromorphs characteristic of T. timopheevii and T. monococcum. Wild tetraploid wheats, T. dicoccoides and T. araraticum, showed partially homologous intraspecific variation of AADH-E with heterozygous triplets E(0.58)/E(0.64) (the commonest), E(0.58)/E(0.71), E(0.45)/E(0.58), E(0.48)/E(0.58) and E(0.56)/E(0.58) recorded. Polyploid goatgrasses of the D-genome group, excepting Ae. cylindrica, are fixed for the common triplet E(0.58)/E(0.64). Ae. cylindrica and polyploid goatgrasses of the C(u)-genome group, excepting Ae. kotschyi, are homozygous for E(0.64). Ae. kotschyi is exceptional, showing fixed heterozygosity for both AADH-E and ADH-A with unique triplets E(0.56)/E(0.64) and A(0.49)/A(0.56).
描述并讨论了 NAD 特异性芳香醇脱氢酶 AADH-E 在小麦和山羊草物种中的进化电泳变异，与之前研究的 NAD 特异性醇脱氢酶 (ADH-A) 和 NADP 依赖性 AADH-B 进行了比较。栽培四倍体硬粒小麦 (T. turgidum s. l.) 和六倍体面包小麦 (T. aestivum s. l.) 均固定为杂合三联体 E(0.58)/E(0.64)。最慢的同工酶 E(0.58)由 T. aestivum cv. 的 6AL 染色体臂上的同系等位基因控制。Chinese Spring'，并存在于所有二倍体小麦、T. monococcum s. Str.、T. boeoticum s. l. 和 T. urartu 中。最快的同工酶 E(0.64) 可能由面包小麦的 B-和 D-基因组同系等位基因控制，是二倍体山羊草中最常见的异源同工酶，包括 Ae. speltaides 和 Ae. tauschii。四倍体 T. timopheevii s. str. 具有特殊的杂合三联体 E(0.56)/E(0.71)，而六倍体 T. zhukovskyi 表现出与 T. timopheevii 和 T. monococcum 特征电形态的多态性。野生四倍体小麦 T. dicoccoides 和 T. araraticum 表现出与 AADH-E 的部分同源种内变异，具有杂合三联体 E(0.58)/E(0.64)（最常见）、E(0.58)/E(0.71)、E(0.45)/E(0.58)、E(0.48)/E(0.58) 和 E(0.56)/E(0.58) 记录。除 Ae. cylindrica 外，D 基因组组的多倍体山羊草固定为常见的三联体 E(0.58)/E(0.64)。Ae. cylindrica 和 C(u)-基因组组的多倍体山羊草，除 Ae. kotschyi 外，均为 E(0.64)纯合子。Ae. kotschyi 是例外，它对 AADH-E 和 ADH-A 均表现出固定的杂合性，具有独特的三联体 E(0.56)/E(0.64)和 A(0.49)/A(0.56)。
