Department of Biology, University, 84112, Salt Lake City, UT, USA.
Plant Cell Rep. 1981 Dec;1(2):48-51. doi: 10.1007/BF00269269.
Following ultraviolet mutagenesis, we have isolated two Na(+) dependent variants from haploid suspension cultures of soybean cells. No selection was used in the isolation procedure. These cells cannot grow in 1 mM Na(+) but can grow in 17 mM Na(+). The strains also grow in 33 or 65 mM Na(+), concentrations in which the parent cell line dies. [(22)Na] was used to measure the accumulation of Na(+) by parent and variant cell lines. Two- to three-fold less Na(+) is retained by the variant cell lines.
经过紫外线诱变，我们从大豆细胞的单倍体悬浮培养物中分离出两种依赖钠离子的变体。在分离过程中没有进行选择。这些细胞不能在 1mM 的钠离子中生长，但可以在 17mM 的钠离子中生长。这些菌株也可以在 33 或 65mM 的钠离子中生长，而在这些浓度下亲本细胞系会死亡。[(22)Na]被用来测量亲本和变体细胞系对钠离子的积累。变体细胞系保留的钠离子减少了两到三倍。
