Mishra Nishi, Tripathi Manoj Kumar, Tiwari Sushma, Tripathi Niraj, Sapre Swapnil, Ahuja Ashok, Tiwari Sharad
Department of Plant Molecular Biology & Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.
Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.
Plants (Basel). 2021 Mar 10;10(3):517. doi: 10.3390/plants10030517.
Soybean ( (L) Merrill) is used in India mostly as a substantial fund of protein and oil, which makes the crop significantly important. Somaclonal variation has been researched as a base of additional variability for drought in soybean. In the present experiment calli/cell clumps/embryoids rose from immature and mature embryonic axis and cotyledons explants were exposed to different concentrations of polyethylene glycol (PEG6000). A discontinuous method proved to be superior as it permitted the calli/embryoids/cell clumps to regain their regeneration competence. A total of 64 (12.21%) plantlets of genotype JS335 and 78 (13.13%) of genotype JS93-05 were regenerated after four consequent subcultures on the selection medium with an effective lethal concentration of 20% PEG6000, and proliferated calli/embryoids/cell clumps were further subcultured on Murashige and Skoog regeneration medium supplemented with 0.5 mgL each of α-napthalene acetic acid (NAA), 6-benzyladenine (BA) and Kinetin (Kn), 20.0 gL sucrose and 7.5 gL agar. Putative drought-tolerant plantlets were acquired from genotype JS93-05 (38) in more numbers compared to genotype JS335 (26). Random decamer primers confirmed the presence of variability between mother plants and regenerated plants from both the genotypes. Since these plantlets recovered from tolerant calli/embryoids/cell clumps selected from the medium supplemented with PEG6000, the possibility exists that these plants may prove to be tolerant against drought stress.
大豆（(L) Merrill）在印度主要作为蛋白质和油的重要来源，这使得该作物具有重要意义。体细胞克隆变异已被研究作为大豆干旱额外变异的基础。在本实验中，从不成熟和成熟胚轴及子叶外植体诱导产生的愈伤组织/细胞团/胚状体，被暴露于不同浓度的聚乙二醇（PEG6000）。一种间断方法被证明更优，因为它能使愈伤组织/胚状体/细胞团恢复其再生能力。在有效致死浓度为20% PEG6000的选择培养基上连续继代培养4次后，基因型JS335共再生出64株（12.21%）植株，基因型JS93 - 05共再生出78株（13.13%）植株，增殖的愈伤组织/胚状体/细胞团在添加了0.5 mg/L的α - 萘乙酸（NAA）、6 - 苄基腺嘌呤（BA）和激动素（Kn）、20.0 g/L蔗糖和7.5 g/L琼脂的Murashige和Skoog再生培养基上进一步继代培养。与基因型JS335（26株）相比，从基因型JS93 - 05获得了更多数量（38株）的假定耐旱植株。随机十聚体引物证实了两种基因型的母本植株与再生植株之间存在变异。由于这些植株是从添加PEG6000的培养基中筛选出的耐旱愈伤组织/胚状体/细胞团再生而来，所以这些植株有可能被证明对干旱胁迫具有耐受性。
