pH 值和溶酶体提取物对大鼠肝金属硫蛋白（Cd-硫蛋白）的影响。

The effect of pH and lysosomal extract on rat liver metallothionein (Cd-thionein).

机构信息

Department of Veterinary Pathology, Faculty of Veterinary Medicine, State University, PO Box 80.158, 3508 TD, Utrecht, The Netherlands.

出版信息

Biol Trace Elem Res. 1985 Feb;7(1):49-54. doi: 10.1007/BF02916546.

DOI:10.1007/BF02916546

PMID:24258874

Abstract

Was studied in vitro. The(109)Cd-labeled protein was isolated by gel filtration and incubated with a lysosomal extract from the same source. No degradation was seen when the pH of the incubation medium was 5 or higher, whereas the degradation of Cd-thionein was completed in 2 h at a pH of 4.5. Dissociation of Cd(2+) from thionein, which takes place to a fair extent at a pH below 5, appears necessary before the enzymatic breakdown of the thionein moiety can take place.

摘要

进行了体外研究。用凝胶过滤法分离（109）Cd 标记的蛋白质，并与同一来源的溶酶体提取物孵育。当孵育介质的 pH 值为 5 或更高时，未观察到降解，而 Cd-硫氧还蛋白在 pH 值为 4.5 时在 2 小时内完全降解。在硫氧还蛋白部分的酶促分解发生之前，似乎需要在 pH 值低于 5 的情况下相当程度地发生 Cd(2+)与硫氧还蛋白的解离。