肠胃外给予锌后肝锌硫蛋白的降解
Degradation of hepatic zinc-thionein after parenteral zinc administration.
作者信息
Feldman S L, Cousins R J
出版信息
Biochem J. 1976 Dec 15;160(3):583-8. doi: 10.1042/bj1600583.
Abstract
A low-molecular-weight protein, zinc-thionein, a metallothionein, was implicated as having a regulatory function in zinc metabolism. The half-life (t 1/2) of hepatic zinc-thionein was determined by pulse-labelling with either L-[35S] cystine and/or 65Zn. In two experiments with L-[35S]cystine, the t 1/2 of zinc-thionein was 18h and 19h. Most of the soluble 35S-labelled hepatic proteins had a t 1/2 of 4 days. The t 1/2 of zinc-thionein calculated by using 65Zn was 20h. The close similarity between the calculated and measured t 1/2 values for zinc-thionein suggests that release of Zn2+ from zinc-thionein probably occurs simultaneously with degradation of the protein moiety.
摘要
一种低分子量蛋白质——锌硫蛋白，属于金属硫蛋白，被认为在锌代谢中具有调节功能。通过用L-[35S]胱氨酸和/或65Zn进行脉冲标记来测定肝脏锌硫蛋白的半衰期（t1/2）。在两项使用L-[35S]胱氨酸的实验中，锌硫蛋白的t1/2分别为18小时和19小时。大多数可溶性35S标记的肝脏蛋白质的t1/2为4天。用65Zn计算得出的锌硫蛋白的t1/2为20小时。锌硫蛋白计算值与测量值之间的紧密相似性表明，锌从锌硫蛋白中的释放可能与蛋白质部分的降解同时发生。
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