Institute of Genetics, University of Amsterdam, Kruislaan 318, NL-1098, SM Amsterdam, The Netherlands.
Theor Appl Genet. 1984 May;68(1-2):25-8. doi: 10.1007/BF00252306.
Two alleles of the structural gene prxA from Petunia, prxA6 and prxA7, could be identified by their differential temporal expression. The alleles prxA6 and prxA7 code for peroxidases with a similar electrophoretic mobility as the products of the previously described alleles prxA1 and prxA5, respectively. The former two alleles differ in that they have a different temporal expression with regard to the temporal expression of the allele prxA2. Crossing experiments indicated that the mutations involved are (cisacting) internal site mutations. In the case of the allele prxA6, the experiments indicated a difference with respect to the allele prxA1 in responsiveness to the action of a trans-acting factor.
通过对其时间表达的差异,从矮牵牛中鉴定出结构基因 prxA 的两个等位基因 prxA6 和 prxA7。等位基因 prxA6 和 prxA7 分别编码与先前描述的等位基因 prxA1 和 prxA5 产物具有相似电泳迁移率的过氧化物酶。前两个等位基因的不同之处在于它们在等位基因 prxA2 的时间表达方面具有不同的时间表达。杂交实验表明,所涉及的突变是(顺式作用)内部位点突变。在等位基因 prxA6 的情况下,实验表明其对反式作用因子作用的反应性与等位基因 prxA1 存在差异。