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矮牵牛过氧化物酶同工酶的遗传学研究:9. 对 prxA 等位基因差异表达的免疫学研究。

Genetics of the peroxidase isoenzymes in Petunia : 9. Immunological investigation into differential expression of prxA alleles.

机构信息

Institute of Genetics, University of Amsterdam, Kruislaan 318, NL-1098, SM Amsterdam, The Netherlands.

出版信息

Theor Appl Genet. 1984 Jun;68(3):265-8. doi: 10.1007/BF00266901.

Abstract

Antibodies were raised against the peroxidases encoded by the allele prxA1 to determine the specific activities of the peroxidases encoded by the alleles prxA1, prxA2, prxA3, and prxA5. The results from double diffusion experiments indicated that all peroxidases encoded by the four alleles are antigenically identical. By rocket immuno electrophoresis it was shown that the peroxidases encoded by the alleles prxA1, prxA2, prxA3, and prxA5 have different specific activities. The results presented are discussed in relation to differential expression of the alleles involved.

摘要

针对等位基因 prxA1 编码的过氧化物酶产生了抗体,以确定等位基因 prxA1、prxA2、prxA3 和 prxA5 编码的过氧化物酶的特异性活性。双扩散实验的结果表明,这四个等位基因编码的所有过氧化物酶在抗原上都是相同的。通过火箭免疫电泳表明,等位基因 prxA1、prxA2、prxA3 和 prxA5 编码的过氧化物酶具有不同的特异性活性。所呈现的结果将与涉及的等位基因的差异表达相关联进行讨论。

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