黑色素瘤中硫酸软骨素蛋白聚糖4（CSPG4）的检测

Detection of chondroitin sulfate proteoglycan 4 (CSPG4) in melanoma.

作者信息

Wang Yangyang, Sabbatino Francesco, Wang Xinhui, Ferrone Soldano

机构信息

Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.

出版信息

Methods Mol Biol. 2014;1102:523-35. doi: 10.1007/978-1-62703-727-3_28.

DOI:10.1007/978-1-62703-727-3_28

PMID:24258997

Abstract

The tumor antigen chondroitin sulfate proteoglycan 4 (CSPG4) appears to be a useful biomarker to identify melanoma cells and an attractive target to apply antibody-based immunotherapy for the treatment of melanoma. Here we described the reverse transcription-polymerase chain reaction (RT-PCR) method and the immunohistochemical (IHC) staining method to detect the expression of CSPG4 in melanoma cells and tissues.

摘要

肿瘤抗原硫酸软骨素蛋白聚糖4（CSPG4）似乎是一种用于识别黑色素瘤细胞的有用生物标志物，也是应用基于抗体的免疫疗法治疗黑色素瘤的一个有吸引力的靶点。在此，我们描述了逆转录聚合酶链反应（RT-PCR）方法和免疫组织化学（IHC）染色方法，以检测黑色素瘤细胞和组织中CSPG4的表达。