通过光声血流测定法检测循环肿瘤细胞。

Detection of circulating tumor cells by photoacoustic flowmetry.

作者信息

Weight Ryan M, Viator John A

机构信息

Drexel University College of Medicine, Hahnemann University Hospital, Philadelphia, PA, USA.

出版信息

Methods Mol Biol. 2014;1102:655-63. doi: 10.1007/978-1-62703-727-3_35.

DOI:10.1007/978-1-62703-727-3_35

PMID:24259004

Abstract

Detection of circulating tumor cells (CTCs) in human blood and lymph systems has the potential to aid clinical decision making in the treatment of cancer (Cristofanilli et al. New Engl J Med 351:781-791, 2004; Check Cap Today 19:1.76-1.86, 2005; Braun and Naume J Clin Oncol 8:1623-1626, 2005). The presence of CTCs may signify the onset of metastasis, indicate relapse, or may be used to monitor disease progression. We built and tested a photoacoustic flowmetry system for detecting circulating melanoma cells (CMCs) by exploiting the broadband absorption spectrum of melanin within CMCs. The device was tested on cultured melanoma cells in saline suspension, melanoma cells spiked in human blood, and in a Stage IV melanoma patient. The device showed a detection threshold of a single pigmented melanoma cell from culture. Results show the potential to assay blood samples from healthy and metastatic patients for the presence of cancerous melanoma providing a method for cancer screening.

摘要

检测人体血液和淋巴系统中的循环肿瘤细胞（CTC）有助于癌症治疗的临床决策（克里斯托法尼利等人，《新英格兰医学杂志》351:781 - 791，2004年；《今日检查帽》19:1.76 - 1.86，2005年；布劳恩和瑙梅，《临床肿瘤学杂志》8:1623 - 1626，2005年）。CTC的存在可能意味着转移的开始、表明复发，或者可用于监测疾病进展。我们构建并测试了一种光声血流测量系统，通过利用循环黑色素瘤细胞（CMC）中黑色素的宽带吸收光谱来检测CMC。该设备在盐溶液悬浮培养的黑色素瘤细胞、添加到人体血液中的黑色素瘤细胞以及一名IV期黑色素瘤患者身上进行了测试。该设备显示出能检测出培养物中单个色素沉着黑色素瘤细胞的检测阈值。结果表明，有可能对健康和转移性患者的血液样本进行检测，以确定是否存在癌性黑色素瘤，从而提供一种癌症筛查方法。