Weight Ryan M, Dale Paul S, Viator John A
Kansas City University of Medicine and Biosciences, Kansas City, Missouri, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:106-9. doi: 10.1109/IEMBS.2009.5335145.
Detection of circulating tumor cells (CTC's) in human blood and lymph systems has the potential to aid clinical decision making in the treatment of cancer. The presence of CTC's may signify the onset of metastasis, indicate relapse, or may be used to monitor disease progression. A photoacoustic flowmetry system was designed and tested for detecting circulating melanoma cells (CMC's) by exploiting the broadband absorption spectrum of melanin within CMC's. The device was tested on cultured melanoma cells in saline suspension and in a Stage IV melanoma patient. The device showed a detection threshold of a single melanotic melanoma cell from culture. Transient photoacoustic events were detected in a sample derived from a Stage IV melanoma patient that corresponded to particles passing through the laser beam path, indicating the presence of single melanoma cells in the human circulatory system.
在人体血液和淋巴系统中检测循环肿瘤细胞（CTC）有助于癌症治疗的临床决策。CTC的存在可能意味着转移的开始、复发的迹象，或者可用于监测疾病进展。设计并测试了一种光声血流测量系统，通过利用CMC中黑色素的宽带吸收光谱来检测循环黑色素瘤细胞（CMC）。该设备在盐溶液悬浮培养的黑色素瘤细胞和一名IV期黑色素瘤患者身上进行了测试。该设备显示出从培养物中检测单个黑色素性黑色素瘤细胞的阈值。在一名IV期黑色素瘤患者的样本中检测到了瞬态光声事件，这些事件与穿过激光束路径的颗粒相对应，表明人体循环系统中存在单个黑色素瘤细胞。
