Liljemalm Rickard, Nyberg Tobias
School of Technology and Health, Royal Institute of Technology, Alfred Nobels Allé 10, 141 52, Huddinge, Sweden,
Ann Biomed Eng. 2014 Apr;42(4):822-32. doi: 10.1007/s10439-013-0940-1. Epub 2013 Nov 21.
The response of cells and tissues to elevated temperatures is highly important in several research areas, especially in the area of infrared neural stimulation. So far, only the heat response of neurons has been considered. In this study, primary rat astrocytes were exposed to infrared laser pulses of various pulse lengths and the resulting cell morphology changes and cell migration was studied using light microscopy. By using a finite element model of the experimental setup the temperature distribution was simulated and the temperatures and times to induce morphological changes and migration were extracted. These threshold temperatures were used in the commonly used first-order reaction model according to Arrhenius to extract the kinetic parameters, i.e., the activation energy, E a, and the frequency factor, A c, for the system. A damage signal ratio threshold was defined and calculated to be 6% for the astrocytes to change morphology and start migrating.
细胞和组织对温度升高的反应在多个研究领域中非常重要，尤其是在红外神经刺激领域。到目前为止，仅考虑了神经元的热反应。在本研究中，将原代大鼠星形胶质细胞暴露于不同脉冲长度的红外激光脉冲下，并使用光学显微镜研究由此产生的细胞形态变化和细胞迁移。通过使用实验装置的有限元模型模拟温度分布，并提取诱导形态变化和迁移的温度和时间。这些阈值温度用于根据阿伦尼乌斯常用的一级反应模型来提取系统的动力学参数，即活化能Ea和频率因子Ac。定义并计算出星形胶质细胞改变形态并开始迁移的损伤信号比阈值为6%。
