Nonaka Koji, Akiyama Junichi, Tatsuta Naomi, Une Satsuki, Ito Kenichi, Ogaya Shinya, Kataoka Masataka, Iwata Akira, Okuda Kuniharu
Department of Physical Therapy, Osaka Prefecture University.
J Phys Ther Sci. 2013 Jun;25(6):709-11. doi: 10.1589/jpts.25.709. Epub 2013 Jul 23.
[Purpose] We reported that carbon dioxide (CO2) water bathing accelerates skeletal muscle regeneration; however, the underlying mechanism was unclear. MyoD and myogenin play roles in muscle regeneration, and the purpose of this study was to determine changes in MyoD and myogenin caused by CO2 water bathing after injury. [Subjects] Sixteen female Wistar rats (n = 4 per group) were used. [Methods] The rats were divided into four groups: no-injury (NI), injury (IC), injury + tap water bathing (ITW), and injury + CO2 water bathing (ICO2). Muscle injury was induced by injection of bupivacaine hydrochloride into the left tibial anterior (TA) muscles. Tap water and CO2 (1,000 ppm) water bathing were performed at 37 °C for 30 minutes once a day. The left TA muscles were removed 4 days after injury, and the expressions of MyoD and myogenin were measured. [Results] MyoD and myogenin were increased in the IC, ITW, and ICO2 groups compared with the NI group. Although the MyoD level was similar in the IC, ITW, and ICO2 groups, myogenin increased more in the ICO2 group than in the IC and ITW groups. [Conclusion] CO2 water bathing after muscle injury appears to induce an increase in the expression of myogenin.
[目的] 我们曾报道二氧化碳（CO₂）水浴可加速骨骼肌再生；然而，其潜在机制尚不清楚。MyoD和肌细胞生成素在肌肉再生中发挥作用，本研究的目的是确定损伤后CO₂水浴引起的MyoD和肌细胞生成素的变化。[对象] 使用16只雌性Wistar大鼠（每组n = 4）。[方法] 将大鼠分为四组：未损伤组（NI）、损伤组（IC）、损伤 + 自来水浴组（ITW）和损伤 + CO₂水浴组（ICO₂）。通过向左侧胫骨前肌注射盐酸布比卡因诱导肌肉损伤。自来水浴和CO₂（1000 ppm）水浴在37°C下每天进行一次，每次30分钟。损伤后4天切除左侧胫骨前肌，测量MyoD和肌细胞生成素的表达。[结果] 与NI组相比，IC组、ITW组和ICO₂组的MyoD和肌细胞生成素增加。尽管IC组、ITW组和ICO₂组的MyoD水平相似，但ICO₂组的肌细胞生成素比IC组和ITW组增加得更多。[结论] 肌肉损伤后进行CO₂水浴似乎可诱导肌细胞生成素表达增加。
