Hatade T, Takeuchi K, Fujita N, Arakawa T, Miki A
Department of Physical Therapy, Faculty of Health Sciences, Kobe University School of Medicine, 7-10-2 Tomogaoka, Sumaku, Kobe, 654-0142, Japan.
J Musculoskelet Neuronal Interact. 2014 Sep;14(3):325-33.
Heat stress could promote skeletal muscle regeneration. But, in the regeneration process, effects of heat stress on myogenic cells and the regulating factors is unknown. Therefore, Influences of heat stress soon after injury on distribution of the myogenic cells and chronological changes in expression of MyoD and myogenin were examined. The first peak of MyoD expression was temporally correlated with the time when proliferating satellite cells began to appear, and the rapid decline of the MyoD expression from the first peak, with the appearance time of myoblasts, respectively in both the non-Heat and Heat groups. The first peak of myogenin expression was temporally correlated with the time when multinuclear cells began to form in the both groups. Due to the heat stress, proliferation and differentiation of myogenic cells and chronological changes in these factors were accelerated one day earlier than in the non-Heat group. As MyoD and myogenin are regulating factor of proliferation and differentiation, heat stress soon after the muscle injury could accelerate the proliferation and differentiation of myogenic cells and the expression of their regulating factors MyoD and myogenin.
热应激可促进骨骼肌再生。但是，在再生过程中，热应激对成肌细胞及其调控因子的影响尚不清楚。因此，研究了损伤后立即施加的热应激对成肌细胞分布以及MyoD和肌细胞生成素表达的时间变化的影响。在非热组和热组中，MyoD表达的第一个峰值在时间上分别与增殖性卫星细胞开始出现的时间以及成肌细胞出现时MyoD表达从第一个峰值迅速下降的时间相关。在两组中，肌细胞生成素表达的第一个峰值在时间上与多核细胞开始形成的时间相关。由于热应激，成肌细胞的增殖和分化以及这些因子的时间变化比非热组提前一天加速。由于MyoD和肌细胞生成素是增殖和分化的调控因子，肌肉损伤后立即施加的热应激可加速成肌细胞的增殖和分化以及其调控因子MyoD和肌细胞生成素的表达。
