Research Institute for Agriculture and Life Science, Seoul National University, Seoul, Republic of Korea ; WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
PLoS One. 2013 Nov 18;8(11):e80226. doi: 10.1371/journal.pone.0080226. eCollection 2013.
The yellow fever mosquito, Aedes aegypti, is a vector for transmitting dengue fever and yellow fever. In this study, we assessed the histopathological and molecular effects of pellitorine, an isobutylamide alkaloid, on the third instar of Ae. aegypti larvae. At 5 mg/l concentration of pellitorine, the whole body of the treated larvae became dark in color, particularly damaged thorax and abdominal regions. Pellitorine was targeted mainly on midgut epithelium and anal gills, indicating variably dramatic degenerative responses of the midgut through a sequential epithelial disorganization. The anterior and posterior midgut was entirely necrosed, bearing only gut lumen residues inside the peritrophic membranes. Pellitorine caused comprehensive damage of anal gill cells and branches of tracheole and debris was found in hemolymph of the anal gills. RT-PCR analysis indicates that the compound inhibited gene expression encoding V-type H(+)-ATPase and aquaporine 4 after treatment with 2.21 mg/l pellitorine. These results verify that pellitorine merits further study as a potential larvicide with a specific target site and a lead molecule for the control of mosquito populations.
埃及伊蚊是传播登革热和黄热病的媒介。在这项研究中，我们评估了胡椒酰胺生物碱胡椒乙胺对埃及伊蚊三龄幼虫的组织病理学和分子影响。在 5mg/L 浓度的胡椒乙胺作用下，处理后的幼虫全身呈深色，尤其是胸部和腹部受损严重。胡椒乙胺主要靶向中肠上皮和肛须，表明中肠通过连续的上皮组织紊乱发生了不同程度的退行性反应。前、后中肠完全坏死，仅在围食膜内残留肠腔内容物。胡椒乙胺导致肛须细胞和气管分支的全面损伤，并且在肛须血淋巴中发现了碎片。RT-PCR 分析表明，该化合物抑制了处理后编码 V 型 H(+)-ATP 酶和水通道蛋白 4 的基因表达。这些结果证实，胡椒乙胺是一种具有特定靶标位点的潜在杀虫剂，是控制蚊群的先导分子，值得进一步研究。
