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从野外RNA样本中发现新型病毒并进行基因组重建，揭示了双翅目宿主中高度分化的病毒。

Novel virus discovery and genome reconstruction from field RNA samples reveals highly divergent viruses in dipteran hosts.

作者信息

Cook Shelley, Chung Betty Y-W, Bass David, Moureau Gregory, Tang Shuoya, McAlister Erica, Culverwell C Lorna, Glücksman Edvard, Wang Hui, Brown T David K, Gould Ernest A, Harbach Ralph E, de Lamballerie Xavier, Firth Andrew E

机构信息

Department of Life Sciences, Natural History Museum, London, United Kingdom.

出版信息

PLoS One. 2013 Nov 18;8(11):e80720. doi: 10.1371/journal.pone.0080720. eCollection 2013.

DOI:10.1371/journal.pone.0080720
PMID:24260463
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3832450/
Abstract

We investigated whether small RNA (sRNA) sequenced from field-collected mosquitoes and chironomids (Diptera) can be used as a proxy signature of viral prevalence within a range of species and viral groups, using sRNAs sequenced from wild-caught specimens, to inform total RNA deep sequencing of samples of particular interest. Using this strategy, we sequenced from adult Anopheles maculipennis s.l. mosquitoes the apparently nearly complete genome of one previously undescribed virus related to chronic bee paralysis virus, and, from a pool of Ochlerotatus caspius and Oc. detritus mosquitoes, a nearly complete entomobirnavirus genome. We also reconstructed long sequences (1503-6557 nt) related to at least nine other viruses. Crucially, several of the sequences detected were reconstructed from host organisms highly divergent from those in which related viruses have been previously isolated or discovered. It is clear that viral transmission and maintenance cycles in nature are likely to be significantly more complex and taxonomically diverse than previously expected.

摘要

我们研究了从野外采集的蚊子和摇蚊（双翅目）中测序得到的小RNA（sRNA）是否可以作为一系列物种和病毒组内病毒流行率的替代特征，利用从野生捕获标本中测序得到的sRNA，为特定感兴趣样本的总RNA深度测序提供信息。采用这种策略，我们从成年黄斑按蚊复合体蚊子中测序得到了一种与慢性蜜蜂麻痹病毒相关的此前未描述病毒的近乎完整基因组，并且从一窝里海伊蚊和骚扰伊蚊蚊子中测序得到了一个近乎完整的昆虫双RNA病毒基因组。我们还重建了与至少其他九种病毒相关的长序列（1503 - 6557个核苷酸）。至关重要的是，检测到的几个序列是从与先前分离或发现相关病毒的宿主生物高度不同的宿主生物中重建出来的。显然，自然界中的病毒传播和维持周期可能比先前预期的要复杂得多，并且在分类学上也更加多样化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/babb/3832450/7de7d4dbac24/pone.0080720.g013.jpg
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2
PRICE: software for the targeted assembly of components of (Meta) genomic sequence data.PRICE：用于（元）基因组序列数据的组件靶向组装的软件。
G3 (Bethesda). 2013 May 20;3(5):865-80. doi: 10.1534/g3.113.005967.
3
Presence of entomobirnaviruses in Chinese mosquitoes in the absence of Dengue virus co-infection.在中国蚊虫中存在昆虫双 RNA 病毒，而不存在登革热病毒共同感染。
探索圣保罗动物园内蚊子病毒群落动态：深入了解蚊子-病毒-环境相互作用。
Front Cell Infect Microbiol. 2025 Jan 10;14:1496126. doi: 10.3389/fcimb.2024.1496126. eCollection 2024.
4
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5
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6
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10
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