Department of Pathology, The University of Texas Medical Branch, Galveston, TX 77555-0609, USA.
Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0609, USA.
Viruses. 2020 Mar 31;12(4):390. doi: 10.3390/v12040390.
This report describes and characterizes a novel entomobirnavirus, designated Port Bolivar virus (PTBV), that was isolated from a pool of mosquitoes collected in a saltwater marsh in East Texas, USA. Full genome sequencing and phylogenetic analyses indicate that PTBV is distinct but genetically related to Drosophila X virus and mosquito X virus, which are assigned to species in the genus , family . PTBV produced cytopathic effect (CPE) in cultures of mosquito (C6/36) cells, but not in Vero cell cultures. Ultrastructural studies of PTBV in infected C6/36 cells demonstrated unenveloped virus particles about 55 nm in diameter.
本报告描述并分析了一种新型的昆虫双 RNA 病毒，命名为波比瓦尔港病毒（PTBV），它是从美国德克萨斯州东海岸一个盐水沼泽中采集的蚊子群中分离出来的。全基因组测序和系统进化分析表明，PTBV 是一种独特的病毒，但与果蝇 X 病毒和蚊子 X 病毒在遗传上相关，这两种病毒被归入科的属。PTBV 在蚊子（C6/36）细胞的培养物中产生细胞病变效应（CPE），但在 Vero 细胞培养物中不产生。在感染的 C6/36 细胞中对 PTVB 进行的超微结构研究表明，该病毒有直径约为 55nm 的无包膜病毒颗粒。
