Alkan S S, Braun D G
Ciba Found Symp. 1986;119:264-78. doi: 10.1002/9780470513286.ch15.
The epitopes of six recombinant human interferon alpha (IFN alpha) subtypes have been analysed using 22 monoclonal antibodies (MAbs) obtained from different sources. The IFN alpha subtype specificity of each MAb was determined by a combined immunoprecipitation-bioassay. Eight different epitopes were identified; the number of epitopes on a given IFN alpha subtype varied between four and eight. Each subtype possessed a unique combination of epitopes. Using the best pair of monoclonal antibodies, predicted from epitope mapping studies, subtype-specific two-site (tandem) assays were developed. It was observed that some non-cross-reactive MAbs influenced each other's binding, indicating the flexible nature of IFN molecules. Competitive radioimmunoassay and the combined immunoprecipitation-bioassay were used to identify a common epitope, present on IFN alpha-A, B, C, F and J but not on IFN alpha-D. In neutralization studies, all MAbs that identified this common epitope inhibited the antiviral activity of all IFN alpha molecules tested. It was concluded that the epitope is located within the receptor-binding region of IFN molecules and is important for biological activity. A tentative localization of the common epitope and the other identified epitopes is proposed.
利用从不同来源获得的22种单克隆抗体（MAb）对六种重组人α干扰素（IFNα）亚型的表位进行了分析。通过免疫沉淀 - 生物测定相结合的方法确定了每种MAb的IFNα亚型特异性。鉴定出了八个不同的表位；给定IFNα亚型上的表位数量在四个到八个之间变化。每种亚型都具有独特的表位组合。利用从表位图谱研究中预测出的最佳单克隆抗体对，开发了亚型特异性双位点（串联）测定法。观察到一些非交叉反应性MAb相互影响彼此的结合，这表明IFN分子具有灵活性。采用竞争性放射免疫测定法和免疫沉淀 - 生物测定相结合的方法鉴定出一个共同表位，该表位存在于IFNα - A、B、C、F和J上，但不存在于IFNα - D上。在中和研究中，所有识别该共同表位的MAb均抑制了所有测试的IFNα分子的抗病毒活性。得出的结论是，该表位位于IFN分子的受体结合区域内，对生物活性很重要。提出了该共同表位和其他已识别表位的初步定位。
