Chahota R K, Mukai Y, Chaudhary H K, Kishore Naval, Sharma T R
Department of Agricultural Biotechnology, CSK Himachal Pradesh Agricultural University, Palampur- 176 062 (HP) India.
Comp Cytogenet. 2011 Nov 9;5(4):345-53. doi: 10.3897/CompCytogen.v5i4.965. eCollection 2011.
The fluorescent in situ hybridization (FISH) technique has been applied to somatic chromosomes in the medicinally important species, Bunium persicum, to elucidate its karyotypes. The bicolour FISH technique involving 18S-5.8S-26S and 5S ribosomal RNA genes as probes was used to assign physical localization and measurement of rDNA sites on homologous pairs of chromosomes. The two 18S-5.8S-26S rRNA gene sites were at the terminal regions of the short arms of the chromosomes 1 and 2 involving NOR region of chromosome 1. The 5S rDNA sites were found on subtelomeric region of the long arm of the chromosome number 5 and at interstitial regions of the short arm of chromosome 7. Based on direct visual analysis of chromosome length, morphology and position of FISH signals, a pioneer attempt has been made to construct metaphase karyotype in Bunium persicum, an endangered medicinal plant of North Western Himalayas.
荧光原位杂交（FISH）技术已应用于重要药用植物波斯孜然芹（Bunium persicum）的体细胞染色体，以阐明其核型。采用双色FISH技术，以18S - 5.8S - 26S和5S核糖体RNA基因为探针，对同源染色体对上的rDNA位点进行物理定位和测量。两个18S - 5.8S - 26S rRNA基因位点位于染色体1和2短臂的末端区域，涉及染色体1的核仁组织区（NOR）。5S rDNA位点位于第5号染色体长臂的亚端粒区域和第7号染色体短臂的中间区域。基于对染色体长度、形态和FISH信号位置的直接视觉分析，首次尝试构建喜马拉雅西北部濒危药用植物波斯孜然芹的中期核型。
