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铲鼻鲶鱼(Hemisorubim platyrhynchos (瓦朗谢讷, 1840))的细胞遗传学,铲鼻鲶鱼是一种在南美河流中广泛分布的物种(鲇形目,油鲶科)。

Cytogenetics of the Porthole Shovelnose Catfish, Hemisorubim platyrhynchos (Valenciennes, 1840) (Siluriformes, Pimelodidae), a widespread species in South American rivers.

作者信息

Swarça Ana Cláudia, Sanchez Sebastian, Dias Ana Lucia, Fenocchio Alberto Sergio

机构信息

Departamento de Histologia, CCB, Universidade Estadual de Londrina, CEP 86051-970, Caixa Postal 6001, Londrina, Paraná, Brazil.

出版信息

Comp Cytogenet. 2013 Apr 25;7(2):103-10. doi: 10.3897/CompCytogen.v7i2.4901. eCollection 2013.

Abstract

Hemisorubim platyrhynchos is a medium- to large-sized pimelodid catfish distributed along several river basins of the Neotropical Region, noteworthy for representing an important fishery source. In this work, Hemisorubim platyrhynchos from three isolated populations were cytogenetically analyzed. The karyotype shows a diploid number of 2n=56 chromosomes comprising 22m, 16sm, 10st, 8a (FN=104). NORs detected by AgNO3 were located in the terminal regions of the short arm of a st chromosome pair, as confirmed by CMA3 and FISH using an 18S rDNA probe. C-banding revealed a small amount of heterochromatin in chromosomes, including the NORs, and one biarmed pair that showed conspicuous positive bands on both arms. This fact was also evidenced when using other banding techniques, such as RE (AluI), and indicates that this pair constitutes a species-specific cytogenetic marker.

摘要

扁吻半鲇是一种中型到大型的油鲇科鲶鱼,分布于新热带地区的多个流域,因其是重要的渔业资源而备受关注。在这项研究中,对来自三个孤立种群的扁吻半鲇进行了细胞遗传学分析。其核型显示二倍体数目为2n = 56条染色体,包括22条中着丝粒染色体、16条亚中着丝粒染色体、10条近端着丝粒染色体、8条端着丝粒染色体(臂数 = 104)。经硝酸银检测的核仁组织区位于一对近端着丝粒染色体短臂的末端区域,这一点通过使用18S rDNA探针的CMA3和荧光原位杂交得以证实。C带显示染色体中存在少量异染色质,包括核仁组织区,以及一对双臂染色体,其两条臂均显示明显的阳性带。使用其他显带技术(如RE(AluI))时也证实了这一事实,这表明该对染色体构成了一个物种特异性的细胞遗传学标记。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06fa/3833754/028ecf3d12d8/CompCytogen-007-103-g001.jpg

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