Swarça Ana Claudia, Dias Ana Lucia, Fenocchio Alberto Sergio
Departamento de Histologia, CCB, Universidade Estadual de Londrina. 86051-970, Caixa Postal 6001, Londrina, Paraná, Brazil.
Departamento de Biologia Geral, CCB, Universidade Estadual de Londrina, Caixa Postal 6001, Londrina, Paraná, Brazil.
Comp Cytogenet. 2017 Mar 6;11(1):119-128. doi: 10.3897/CompCytogen.v11i1.11152. eCollection 2017.
The objective of this study was to cytogenetically analyze comparing the findings with other data to infer relationships among Pimelodidae species. The results revealed a diploid number of 2n = 56 and the karyotype composed of 16 metacentric, 20 submetacentric, 6 subtelocentric and 14 acrocentric chromosomes (FN = 98). The Ag-NORs, 18S rDNA and CMA signals were coincident in location occupying the short arm of an acrocentric chromosome pair (23), in a secondary constriction. The 5S rDNA genes were localized near the centromere on the short arms of one submetacentric chromosome pair. C-bands were localized predominantly in the terminal regions of chromosomes, including the AgNORs and a small metacentric pair with a conspicuous positive band on interstitial region. This chromosome pair could be considered a species-specific cytogenetic marker.
本研究的目的是进行细胞遗传学分析，将研究结果与其他数据进行比较，以推断油鲶科物种之间的关系。结果显示二倍体数目为2n = 56，核型由16条中着丝粒染色体、20条亚中着丝粒染色体、6条近端着丝粒染色体和14条端着丝粒染色体组成（臂数 = 98）。银染核仁组织区（Ag-NORs）、18S rDNA和CMA信号在位置上重合，位于一对端着丝粒染色体（23）短臂的一个次缢痕处。5S rDNA基因定位于一对亚中着丝粒染色体短臂的着丝粒附近。C带主要定位于染色体的末端区域，包括AgNORs和一对小的中着丝粒染色体，其在间质区域有明显的阳性带。这对染色体可被视为物种特异性的细胞遗传学标记。
