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估算恶性疟原虫的疟原虫血症:国家室间质评计划的经验。

Estimating the parasitaemia of Plasmodium falciparum: experience from a national EQA scheme.

机构信息

UKNEQAS Parasitology, Department of Clinical Parasitology, Hospital for Tropical Diseases, Mortimer Market, Capper Street, London WC1E 6JB, UK.

出版信息

Malar J. 2013 Nov 22;12:428. doi: 10.1186/1475-2875-12-428.

Abstract

BACKGROUND

To examine performance of the identification and estimation of percentage parasitaemia of Plasmodium falciparum in stained blood films distributed in the UK National External Quality Assessment Scheme (UKNEQAS) Blood Parasitology Scheme.

METHODS

Analysis of performance for the diagnosis and estimation of the percentage parasitaemia of P. falciparum in Giemsa-stained thin blood films was made over a 15-year period to look for trends in performance.

RESULTS

An average of 25% of participants failed to estimate the percentage parasitaemia, 17% overestimated and 8% underestimated, whilst 5% misidentified the malaria species present.

CONCLUSIONS

Although the results achieved by participants for other blood parasites have shown an overall improvement, the level of performance for estimation of the parasitaemia of P. falciparum remains unchanged over 15 years. Possible reasons include incorrect calculation, not examining the correct part of the film and not examining an adequate number of microscope fields.

摘要

背景

检测英国国家外部质量评估计划(UKNEQAS)血液寄生虫学计划中分布的疟原虫血片的寄生虫百分比鉴定和估计的性能。

方法

在 15 年期间,对疟原虫吉姆萨染色薄血片的诊断和估计寄生虫百分比的性能进行了分析,以寻找性能趋势。

结果

平均有 25%的参与者未能估计寄生虫百分比,17%的参与者高估,8%的参与者低估,而 5%的参与者错误识别了存在的疟疾病种。

结论

尽管参与者对其他血液寄生虫的结果显示出总体改善,但在 15 年内,对疟原虫寄生虫百分比的估计性能仍未改变。可能的原因包括计算错误、未检查正确的电影部分以及未检查足够数量的显微镜视野。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1ff/4222811/97f70e83658b/1475-2875-12-428-1.jpg

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