Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, 1960 East-West Road, Honolulu, HI 96822, USA.
Science. 2014 Jan 3;343(6166):69-72. doi: 10.1126/science.1242544. Epub 2013 Nov 21.
The Y chromosome is thought to be important for male reproduction. We have previously shown that, with the use of assisted reproduction, live offspring can be obtained from mice lacking the entire Y chromosome long arm. Here, we demonstrate that live mouse progeny can also be generated by using germ cells from males with the Y chromosome contribution limited to only two genes, the testis determinant factor Sry and the spermatogonial proliferation factor Eif2s3y. Sry is believed to function primarily in sex determination during fetal life. Eif2s3y may be the only Y chromosome gene required to drive mouse spermatogenesis, allowing formation of haploid germ cells that are functional in assisted reproduction. Our findings are relevant, but not directly translatable, to human male infertility cases.
Y 染色体被认为对男性生殖至关重要。我们之前已经表明，通过使用辅助生殖技术，可以从缺失整条 Y 染色体长臂的小鼠中获得活后代。在这里，我们证明，通过使用 Y 染色体仅贡献两个基因（睾丸决定因子 Sry 和精原细胞增殖因子 Eif2s3y）的雄性的生殖细胞，也可以产生活的小鼠后代。Sry 被认为主要在胎儿期的性别决定中发挥作用。Eif2s3y 可能是驱动小鼠精子发生所必需的唯一 Y 染色体基因，允许形成在辅助生殖中起作用的单倍体生殖细胞。我们的发现与人类男性不育病例相关，但不能直接转化。
