Ahmed Emad A, de Rooij Dirk G
Department of Endocrinology, Utrecht University, Utrecht, The Netherlands.
Methods Mol Biol. 2009;558:263-77. doi: 10.1007/978-1-60761-103-5_16.
Spermatogenesis is a cyclic process during which, within each epithelial area, various generations of germ cells undergo a series of developmental steps according to a fixed time schedule. The cycle of the seminiferous epithelium can be subdivided into stages. In the mouse, 12 such stages have been described that can be distinguished from one another by steps in spermatid development. The best way to recognize the stages in seminiferous tubule cross-sections is to use Bouin's-fixed testes of normal mice and sections stained with the Periodic acid Schiff (PAS) technique and hematoxylin. Unfortunately, this is not always possible. Sometimes PAS staining cannot be used, such as when immunohistochemistry is carried out. Moreover, not all germ cell types may be present in some instances, as in young or mutant mice. We summarize here all stage-identifying criteria that can be used in the ideal situation as well as in hematoxylin-only stained sections and/or when germ cell types are missing.
精子发生是一个循环过程，在此过程中，在每个上皮区域内，不同代的生殖细胞按照固定的时间安排经历一系列发育步骤。生精上皮的周期可细分为不同阶段。在小鼠中，已描述了12个这样的阶段，这些阶段可通过精子细胞发育的步骤相互区分。识别生精小管横切面中各阶段的最佳方法是使用正常小鼠经Bouin固定的睾丸以及用高碘酸希夫（PAS）技术和苏木精染色的切片。不幸的是，这并非总是可行的。有时无法使用PAS染色，例如在进行免疫组织化学时。此外，在某些情况下，并非所有生殖细胞类型都可能存在，如在幼年或突变小鼠中。我们在此总结了所有可用于理想情况以及仅用苏木精染色的切片和/或生殖细胞类型缺失时的阶段识别标准。
