Ogasawara H, Fujitani T, Drzewiecki G, Middleton E
J Allergy Clin Immunol. 1986 Aug;78(2):321-8. doi: 10.1016/s0091-6749(86)80083-5.
Studies on hydrogen peroxide (H2O2)-induced histamine release from human basophils indicate that H2O2 is a weak stimulus of histamine release, that the release process is Ca2+ and energy-dependent, and that histamine release is not influenced by theophylline (in keeping with previous observations with rat mast cells). Low concentrations of H2O2 appeared to augment and high concentrations to inhibit histamine release induced by anti-IgE. However, the inhibitory effect of high concentrations of H2O2 were completely abrogated by catalase, which destroys H2O2, and thus indicates that basophils retain immunologic responsivity and are not irreversibly effected by high concentrations of H2O2. Leukocyte suspensions relatively enriched in monocytes, lymphocytes, basophils, neutrophils, and neutrophils plus eosinophils were prepared by Percoll-gradient centrifugation. Anti-IgE stimulated H2O2 formation only in the fraction richest in basophils. Opsonized zymosan, on the other hand, stimulated H2O2 generation in both the basophil and monocyte fractions, indicating activation of both monocytes and basophils by this stimulus. Mixtures of basophil-containing leukocyte suspensions plus purified neutrophils and opsonized zymosan stimulated histamine release in proportion to concomitant generation of H2O2. Addition of catalase reduced histamine release under these conditions, whereas scavengers of other toxic oxygen derivatives (superoxide dismutase, alpha-tocopherol, D-mannitol) had little or no effect on histamine release. These findings suggest that neutrophil-derived H2O2 can cause basophil histamine release in mixed populations of activated leukocytes. Three naturally occurring flavonoids, quercetin, apigenin, and taxifolin (dihydroquercetin) were examined for their effect on anti-IgE-induced histamine release and H2O2 generation in basophil-containing leukocyte suspensions.(ABSTRACT TRUNCATED AT 250 WORDS)
关于过氧化氢（H₂O₂）诱导人嗜碱性粒细胞释放组胺的研究表明，H₂O₂是组胺释放的弱刺激物，释放过程依赖Ca²⁺和能量，且组胺释放不受茶碱影响（与先前对大鼠肥大细胞的观察结果一致）。低浓度的H₂O₂似乎增强而高浓度则抑制抗IgE诱导的组胺释放。然而，高浓度H₂O₂的抑制作用可被过氧化氢酶完全消除，过氧化氢酶可破坏H₂O₂，这表明嗜碱性粒细胞保留免疫反应性，且不会被高浓度H₂O₂不可逆地影响。通过Percoll梯度离心制备了相对富含单核细胞、淋巴细胞、嗜碱性粒细胞、中性粒细胞以及中性粒细胞加嗜酸性粒细胞的白细胞悬液。抗IgE仅在嗜碱性粒细胞最丰富的部分刺激H₂O₂生成。另一方面，调理后的酵母聚糖在嗜碱性粒细胞和单核细胞部分均刺激H₂O₂生成，表明该刺激可激活单核细胞和嗜碱性粒细胞。含嗜碱性粒细胞的白细胞悬液与纯化的中性粒细胞及调理后的酵母聚糖的混合物刺激组胺释放的比例与同时产生的H₂O₂成比例。在此条件下添加过氧化氢酶可减少组胺释放，而其他有毒氧衍生物的清除剂（超氧化物歧化酶、α-生育酚、D-甘露醇）对组胺释放几乎没有影响。这些发现表明，在活化白细胞混合群体中，中性粒细胞衍生的H₂O₂可导致嗜碱性粒细胞组胺释放。研究了三种天然存在的黄酮类化合物槲皮素、芹菜素和紫杉叶素（二氢槲皮素）对含嗜碱性粒细胞的白细胞悬液中抗IgE诱导的组胺释放和H₂O₂生成的影响。（摘要截短于250字）
