Brenner T, Lisak R P, Rostami A, Pleasure D E, Silberberg D H
J Neurosci. 1986 Jul;6(7):1925-33. doi: 10.1523/JNEUROSCI.06-07-01925.1986.
We have produced a monoclonal antibody against myelin basic protein that reacts with astrocytes, oligodendrocytes, and Schwann cells. This antibody was generated by fusion of mouse myeloma cells with spleen cells from BALB/c mice immunized with delipidated white matter from adult rat corpus callosum. The antibody was characterized via solid-phase radioimmunoassay, immunoblot of SDS-PAGE, and by indirect immunofluorescence staining of monolayer cultures containing oligodendrocytes, astrocytes, and Schwann cells. Myelin basic protein (MBP) was shown previously to be present only in myelin producing cells in CNS and PNS (oligodendroglia and Schwann cells) and not in astrocytes. The binding of this monoclonal antibody to all 3 cell types suggests that these cells share a common epitope. This epitope may be related to a common progenitor cell.
我们制备了一种针对髓鞘碱性蛋白的单克隆抗体，该抗体可与星形胶质细胞、少突胶质细胞和施万细胞发生反应。此抗体是通过将小鼠骨髓瘤细胞与用成年大鼠胼胝体脱脂白质免疫的BALB/c小鼠的脾细胞融合而产生的。通过固相放射免疫测定、SDS-PAGE免疫印迹以及对含有少突胶质细胞、星形胶质细胞和施万细胞的单层培养物进行间接免疫荧光染色来对该抗体进行表征。髓鞘碱性蛋白（MBP）先前已被证明仅存在于中枢神经系统和外周神经系统中产生髓鞘的细胞（少突胶质细胞和施万细胞）中，而不存在于星形胶质细胞中。这种单克隆抗体与所有三种细胞类型的结合表明这些细胞共享一个共同的表位。这个表位可能与一个共同的祖细胞有关。
