培养的人及大鼠少突胶质细胞和大鼠雪旺细胞表面没有免疫反应基因相关抗原（Ia）。

Cultured human and rat oligodendrocytes and rat Schwann cells do not have immune response gene associated antigen (Ia) on their surface.

作者信息

Lisak R P, Hirayama M, Kuchmy D, Rosenzweig A, Kim S U, Pleasure D E, Silberberg D H

出版信息

Brain Res. 1983 Dec 19;289(1-2):285-92. doi: 10.1016/0006-8993(83)90029-x.

Abstract

In order to determine if oligodendrocytes or Schwann cells had surface immune response gene associated antigen (Ia), we studied the binding of: (a) mouse monoclonal antibodies to rat Ia, to cultures of rat oligodendrocytes and Schwann cells; and, (b) mouse monoclonal antibodies to human Ia, to cultures of human oligodendrocytes employing radioimmunoassay and indirect immunofluorescence. Cells were identified using phenotypic markers; rabbit anti-galactocerebroside (GalC) for oligodendrocytes; rabbit anti-GalC and rabbit anti-Schwann cell for Schwann cells; rabbit anti-glial fibrillary acidic protein for astrocytes; rabbit anti-fibronectin for fibro-blasts and leptomeningeal cells, and the capacity to ingest latex particles for macrophage-microglia. Ia could not be detected on the surface of oligodendrocytes, Schwann cells, astrocytes, fibroblasts, or leptomeningeal cells. A small number of latex ingesting cells bound anti-Ia even after blocking of surface Fc receptors.

摘要

为了确定少突胶质细胞或施万细胞是否具有表面免疫反应基因相关抗原（Ia），我们进行了以下研究：（a）使用放射免疫测定法和间接免疫荧光法，将小鼠抗大鼠Ia单克隆抗体与大鼠少突胶质细胞和施万细胞培养物进行结合实验；以及（b）将小鼠抗人Ia单克隆抗体与人类少突胶质细胞培养物进行结合实验。利用表型标志物来鉴定细胞；用兔抗半乳糖脑苷脂（GalC）鉴定少突胶质细胞；用兔抗GalC和兔抗施万细胞鉴定施万细胞；用兔抗胶质纤维酸性蛋白鉴定星形胶质细胞；用兔抗纤连蛋白鉴定成纤维细胞和软脑膜细胞，以及利用摄取乳胶颗粒的能力来鉴定巨噬细胞-小胶质细胞。在少突胶质细胞、施万细胞、星形胶质细胞、成纤维细胞或软脑膜细胞表面均未检测到Ia。即使在阻断表面Fc受体后，仍有少量摄取乳胶颗粒的细胞与抗Ia结合。