Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, People's Republic of China ; Department of Pathology, College of Basic Medical Sciences, Shenyang Medical College, Shenyang, People's Republic of China.
PLoS One. 2013 Nov 12;8(11):e79173. doi: 10.1371/journal.pone.0079173. eCollection 2013.
The objective of the current study was to determine the clinical significance of junctional adhesion molecule A (JAM-A) in patients with non-small cell lung cancer (NSCLC) and the biological function of JAM-A in NSCLC cell lines. We showed that JAM-A is predominantly expressed in cell membranes and high expression of JAM-A occurred in 37% of lung tumor specimens compared to corresponding normal tissues. High expression of JAM-A was significantly correlated with TNM stage (P = 0.021), lymph node metastasis (P = 0.007), and decreased overall survival (P = 0.02), In addition, we observed that silencing JAM-A by small interfering RNA inhibited tumor cell proliferation and induced cell cycle arrest at the G1/S boundary. Western blotting analysis revealed that knockdown of JAM-A decreased the protein levels of cyclin D1, CDK4, 6, and P-Rb. Thus, JAM-A plays an important role in NSCLC progression.
本研究旨在探讨细胞间黏附分子 A（JAM-A）在非小细胞肺癌（NSCLC）患者中的临床意义，以及 JAM-A 在 NSCLC 细胞系中的生物学功能。结果表明，JAM-A 主要表达于细胞膜上，与相应的正常组织相比，37%的肺癌组织标本中 JAM-A 呈高表达。JAM-A 的高表达与 TNM 分期（P=0.021）、淋巴结转移（P=0.007）和总生存期缩短显著相关（P=0.02）。此外，我们发现通过小干扰 RNA 沉默 JAM-A 可抑制肿瘤细胞增殖，并诱导细胞周期在 G1/S 期边界停滞。Western blot 分析显示，JAM-A 下调可降低细胞周期蛋白 D1、CDK4、6 和 P-Rb 的蛋白水平。因此，JAM-A 在 NSCLC 的进展中发挥重要作用。
