Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, Maharashtra, India.
J Biomed Nanotechnol. 2013 Dec;9(12):2073-85. doi: 10.1166/jbn.2013.1736.
We studied the in vivo performance of scaffolds consisting of nanofibrous poly(L-lactic acid) (P) and blend of poly(L-lactic acid/gelatin) (PG) prepared by electrospinning and further composited them with hydroxyapatite (HA) via alternate soaking method, to get poly(L-lactic acid)/hydroxyapatite (PH) and poly(L-lactic acid)/gelatin/hydroxyapatite (PGH) scaffolds respectively. The purpose of this study was to assess and compare bone regeneration potential of electrospun P, PG and electrospun-alternate soaked PH and PGH scaffolds using rat as an animal model by creating two 5 mm circular defects in calvaria. The respective scaffolds were implanted into the defects as one side implantation and both side implantation. Defects left empty served as a negative control for one side implantation and as sham control for both side implantations. The outcomes of the scaffold implantation were determined after 6 and 10 weeks by digital radiography, micro-CT, dual-energy X-ray absorptiometry (DEXA) and histological analysis. PGH scaffold regenerated maximum amount of new bone with high bone mineral density (BMD) into the defects and complete closure occurred in just 6 weeks while other scaffolds failed to close the defects completely. PGH group exhibited highest BMD value after 10 weeks. Histological findings showed abundant osteoblasts and initiation of matrix mineralization in HA containing scaffolds. Masson's trichrome staining showed collagen deposition in all scaffold groups except sham control group. Biochemical and haematological parameters were well with in normal range, indicating no infection due to scaffold implantation. These results prove PGH scaffold as a potential biomaterial for bone regenerative medicine.
我们研究了由静电纺丝制备的纳米纤维聚（L-乳酸）（P）和聚（L-乳酸/明胶）（PG）混合物组成的支架的体内性能，并通过交替浸泡法将其与羟基磷灰石（HA）复合，分别得到聚（L-乳酸）/羟基磷灰石（PH）和聚（L-乳酸）/明胶/羟基磷灰石（PGH）支架。本研究的目的是通过在颅骨中创建两个 5 毫米的圆形缺陷，使用大鼠作为动物模型，评估和比较静电纺丝 P、PG 和静电纺丝-交替浸泡 PH 和 PGH 支架的骨再生潜力。将各自的支架植入缺陷中作为单侧植入，以及双侧植入。缺陷的空白部分作为单侧植入的阴性对照，作为双侧植入的假对照。在 6 和 10 周后，通过数字射线照相、微 CT、双能 X 射线吸收法（DEXA）和组织学分析确定支架植入的结果。PGH 支架在 6 周内将最大量的新骨和高骨矿物质密度（BMD）再生到缺陷中，并完全闭合，而其他支架未能完全闭合缺陷。PGH 组在 10 周后表现出最高的 BMD 值。组织学发现，在含有 HA 的支架中存在丰富的成骨细胞和基质矿化的启动。Masson 三色染色显示所有支架组均有胶原蛋白沉积，除了假对照组。生化和血液学参数均在正常范围内，表明由于支架植入没有感染。这些结果证明 PGH 支架是骨再生医学的一种有潜力的生物材料。
