State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China.
Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston Salem, NC 27103, USA.
Int J Nanomedicine. 2019 May 27;14:3929-3941. doi: 10.2147/IJN.S205194. eCollection 2019.
Hierarchical nanofibrous scaffolds are emerging as a promising bone repair material due to their high cell adhesion activity and nutrient permeability. However, the existing method for hierarchical nanofibrous scaffolds fabrication is complicated and not perfectly suitable for further biomedical application in view of both structure and function. In this study, we constructed a hierarchical nanofibrous poly (l-lactic acid)/poly(ε-caprolactone) (PLLA/PCL) scaffold and further evaluated its bone healing ability. The hierarchical PLLA/PCL nanofibrous scaffold (PLLA/PCL) was prepared by one-pot TIPS and then rapidly mineralized at room temperature by an electrochemical deposition technique. After electrode-positioning at 2 V for 2 hrs, a scaffold coated with hydroxyapatite (M-PLLA/PCL) could be obtained. The pore size of the M-PLLA/PCL scaffold was hierarchically distributed so as to match the biophysical structure for osteoblast growth. The M-PLLA/PCL scaffold showed better cell proliferation and osteogenesis activity compared to the PLLA/PCL scaffold. Further in vivo bone repair studies indicated that the M-PLLA/PCL scaffold could accelerate defect healing in 12 weeks. The results of this study implied that the as-prepared hydroxyapatite coated hierarchical PLLA/PCL nanofibrous scaffolds could be developed as a promising material for efficient bone tissue repair after carefully tuning the TIPS and electrodeposition parameters.
分层纳米纤维支架由于其高细胞黏附活性和营养渗透性而成为有前途的骨修复材料。然而，现有的分层纳米纤维支架制造方法比较复杂，从结构和功能两方面来看，并不完全适合进一步的生物医学应用。在本研究中，我们构建了一种分层纳米纤维聚（L-乳酸）/聚（ε-己内酯）（PLLA/PCL）支架，并进一步评估了其骨愈合能力。通过一锅热诱导相分离（TIPS）法制备了分层 PLLA/PCL 纳米纤维支架（PLLA/PCL），然后通过电化学沉积技术在室温下快速矿化。在 2 V 下电沉积 2 小时后，可获得涂覆有羟基磷灰石的支架（M-PLLA/PCL）。M-PLLA/PCL 支架的孔径呈分层分布，以匹配成骨细胞生长的生物物理结构。与 PLLA/PCL 支架相比，M-PLLA/PCL 支架显示出更好的细胞增殖和成骨活性。进一步的体内骨修复研究表明，M-PLLA/PCL 支架可在 12 周内加速缺损愈合。本研究结果表明，所制备的涂覆有羟基磷灰石的分层 PLLA/PCL 纳米纤维支架可通过仔细调整 TIPS 和电沉积参数，开发为一种高效的骨组织修复的有前途的材料。
