Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi, 627 412 Tirunelveli, Tamil Nadu, India.
Pestic Biochem Physiol. 2013 Nov;107(3):369-76. doi: 10.1016/j.pestbp.2013.10.005. Epub 2013 Oct 18.
The culture supernatant of a strain of Bacillus subtilis isolated from soil samples killed larvae of the mosquito Aedes aegypti. The metabolites produced by B. subtilis were characterized using high performance liquid chromatography (HPLC). Mortality rate was dose-dependent for all larval instars of A. aegypti. Log probit analysis (95% confidence level) revealed an LC50 of 1.73 and an LC90 3.71μg/ml. Molecular weights/masses of B. subtilis metabolites were confirmed using SDS-PAGE analysis. B. subtilis metabolites were confirmed using HPLC analysis. We demonstrate that secondary metabolites from B. subtilis have larvicidal activity against A. aegypti and may be suitable for the control of this and other mosquito vectors of human disease. The larvae to the metabolites, significant reduction in the activities of acetylcholinesterse, α-carboxylesterase, and acid phosphatases were recorded.
从土壤样本中分离出的一株枯草芽孢杆菌的培养液上清液可杀死埃及伊蚊幼虫。采用高效液相色谱（HPLC）对枯草芽孢杆菌产生的代谢产物进行了鉴定。对所有埃及伊蚊幼虫龄期，死亡率均与剂量呈正相关。对数概率分析（95%置信水平）显示 LC50 为 1.73μg/ml，LC90 为 3.71μg/ml。采用 SDS-PAGE 分析确定枯草芽孢杆菌代谢产物的分子量/质量。采用 HPLC 分析对枯草芽孢杆菌代谢产物进行了鉴定。我们证明，枯草芽孢杆菌的次生代谢物对埃及伊蚊具有杀幼虫活性，可能适合控制这种和其他蚊媒传播的人类疾病。与代谢物接触后，乙酰胆碱酯酶、α-羧酸酯酶和酸性磷酸酶的活性显著降低。
