Chandy K G, DeCoursey T E, Fischbach M, Talal N, Cahalan M D, Gupta S
Science. 1986 Sep 12;233(4769):1197-200. doi: 10.1126/science.2426784.
The observation that voltage-dependent K+ channels are required for activation of human T lymphocytes suggests that pathological conditions involving abnormal mitogen responses might be reflected in ion channel abnormalities. Gigaohm seal techniques were used to study T cells from MRL/MpJ-lpr/lpr mice; these mice develop generalized lymphoproliferation of functionally and phenotypically abnormal T cells and a disease resembling human systemic lupus erythematosus. The number and predominant type of K+ channels in T cells from these mice differ dramatically from those in T cells from control strains and a congenic strain lacking the lpr gene locus. Thus an abnormal pattern of ion channel expression has now been associated with a genetic defect in cells of the immune system.
电压依赖性钾通道是人类T淋巴细胞激活所必需的这一观察结果表明，涉及异常丝裂原反应的病理状况可能反映在离子通道异常中。采用千兆欧封接技术研究了MRL/MpJ-lpr/lpr小鼠的T细胞；这些小鼠发生功能和表型异常的T细胞的全身性淋巴细胞增殖以及一种类似于人类系统性红斑狼疮的疾病。这些小鼠T细胞中钾通道的数量和主要类型与对照品系以及缺乏lpr基因座的同基因品系T细胞中的钾通道数量和主要类型有显著差异。因此，离子通道表达的异常模式现已与免疫系统细胞中的遗传缺陷相关联。
