Decoursey T E, Chandy K G, Gupta S, Cahalan M D
J Gen Physiol. 1987 Mar;89(3):405-20. doi: 10.1085/jgp.89.3.405.
Using gigohm-seal recording, we studied ion channel expression in resting and activated T lymphocytes from mice. Both the number of channels per cell and the predominant type of K+ channel depend upon the state of activation of the cell. Unstimulated T cells express small numbers of K+ channels, typically a dozen per cell, and are heterogeneous, usually expressing either type n or type l K+ channels (see DeCoursey, T. E., K. G. Chandy, S. Gupta, and M. D. Cahalan. 1987. Journal of General Physiology. 89:379-404). 1 d after stimulation by the murine T cell mitogen concanavalin A, large numbers of type n K+ channels appear in enlarged, activated cells. Type n channels appear in activated cells with a time course consistent with that reported for mitogen-induced enhancement of protein synthesis. Voltage-gated tetrodotoxin-sensitive Na+ channels present in about one-third of unstimulated cells from the MRL-n strain are increased approximately 10-fold after activation.
利用千兆欧封接记录技术，我们研究了小鼠静息和活化T淋巴细胞中的离子通道表达。每个细胞的通道数量和主要的钾离子通道类型均取决于细胞的活化状态。未受刺激的T细胞表达少量钾离子通道，通常每个细胞有十几个，且具有异质性，通常表达n型或l型钾离子通道（见德库西，T.E.，K.G.钱迪，S.古普塔和M.D.卡哈兰。1987年。《普通生理学杂志》。89:379 - 404）。在受到小鼠T细胞有丝分裂原伴刀豆球蛋白A刺激1天后，大量n型钾离子通道出现在增大的活化细胞中。n型通道出现在活化细胞中的时间进程与有丝分裂原诱导的蛋白质合成增强所报道的一致。MRL - n品系约三分之一未受刺激的细胞中存在的电压门控河豚毒素敏感钠离子通道在活化后增加约10倍。
