Linshaw M A, Bauman C A, Welling L W
Am J Physiol. 1986 Aug;251(2 Pt 2):F214-9. doi: 10.1152/ajprenal.1986.251.2.F214.
Proximal convoluted renal tubules (PCT) are difficult to dissect for in vitro study, particularly in newborn animals, and are identified with certainty only when obtained in continuity with their parent glomeruli. We show here that the supravital colloidal dye trypan blue preferentially stains early PCT segments of rabbit in vivo and greatly facilitates their isolation. We also show that trypan blue administration does not adversely affect animal growth rate, kidney weight, or several general measures of kidney function. Using isolated PCT from newborn to adult rabbits, control (unstained) and trypan blue-stained tubules also are found not to differ in lateral membrane surface density, in their ability to maintain normal cell volume or to respond to hypotonic osmotic challenge, and in their swelling rate in isotonic medium after ouabain treatment. We conclude that trypan blue has no adverse effects on PCT cell function while greatly facilitating their isolation for physiological studies.
近端曲管（PCT）难以分离用于体外研究，尤其是在新生动物中，并且只有在与它们的母肾小球连续获得时才能确定识别。我们在此表明，活体染料锥虫蓝在体内优先染色兔的早期PCT段，并极大地促进了它们的分离。我们还表明，给予锥虫蓝不会对动物生长速率、肾脏重量或几种肾脏功能的一般指标产生不利影响。使用从新生兔到成年兔分离的PCT，对照（未染色）和锥虫蓝染色的肾小管在侧膜表面密度、维持正常细胞体积或对低渗渗透挑战作出反应的能力以及哇巴因处理后在等渗介质中的肿胀速率方面也没有差异。我们得出结论，锥虫蓝对PCT细胞功能没有不利影响，同时极大地促进了它们用于生理学研究的分离。
