Ruegg C E
In Vitro Technologies, Inc, Baltimore, Maryland 21227.
J Pharmacol Toxicol Methods. 1994 Jun;31(3):125-33. doi: 10.1016/1056-8719(94)90074-4.
Previous research in animals and humans has demonstrated that many nephrotoxic chemicals induce selective injury within the kidney affecting either renal proximal straight (PST) or proximal convoluted (PCT) tubules. Selective injury has also been observed following in vitro nephrotoxicant exposure to precision-cut renal slices and isolated PCT and PST segments. These in vitro models provide a means of comparing and contrasting basic mechanistic differences which render these segments innately susceptible to nephrotoxicant injury. In this article, methods for preparing precision-cut slices and isolating PST and PCT segments will be reviewed.
此前在动物和人类身上开展的研究表明，许多肾毒性化学物质会在肾脏内引发选择性损伤，影响肾近端直小管（PST）或近端曲小管（PCT）。在体外将肾毒性物质暴露于精密切割的肾切片以及分离出的PCT和PST节段后，也观察到了选择性损伤。这些体外模型提供了一种比较和对比基本机制差异的方法，这些差异使得这些节段天生就易受肾毒性物质损伤。在本文中，将对制备精密切割切片以及分离PST和PCT节段的方法进行综述。
