Liu Kai Shan, Fan Xiao Qin, Zhang Lei, Wen Qiong Na, Feng Ji Hong, Chen Fu Chao, Luo Jun Min, Sun Wan Bang
The Border Armed Police Central Hospital of Guangdong Province, Shenzhen, Guangdong 518023, P.R. China.
College of Life Science and Teaching, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
Mol Med Rep. 2014 Feb;9(2):639-44. doi: 10.3892/mmr.2013.1817. Epub 2013 Nov 20.
The current study aimed to investigate the rejection and survival time of grafted skin, and the changes of Treg cells, interleukin 10 (IL-10) and transforming growth factor-β (TGF-β) in peripheral blood following skin transplantation with recombinant human interleukin-10 (rhIL-10) or cyclosporin A (CsA), as well as the role of IL-10 in immunological rejection mechanisms. A total of 36 rabbits were divided into two groups. The skin of a donor rabbit was transplanted onto the back of one receptor rabbit. Receptors were randomly divided into six groups, including rhIL-10 low-dose (5 µg/kg/d), rhIL-10 high-dose (10 µg/kg/d), CsA low-dose (5 mg/kg/d), CsA high-dose (10 mg/kg/d), rhIL-10 (5 µg/kg/d) and CsA (5 mg/kg/d) and negative control normal saline (NS; 1 ml/d). All groups received intramuscular drug injection for ten days, beginning one day prior to skin transplantation surgery. Following transplantation, each rabbit's peripheral blood was collected at different times. The changes of CD4+CD25+ regulatory T cells, IL-10 and TGF-β were determined by flow cytometry and enzyme-linked immunosorbent assay. When compared with the control group, the rejection and survival times of the experimental groups were longer following skin graft. Compared with the two CsA groups and the control group, the proportion of CD4+CD25+ regulatory T cells of rhIL-10 groups was significantly upregulated on the 4th and 7th days following surgery. However, TGF-β levels were not significantly different. Data suggested that the concentration of IL-10 was positively correlated with the proportion of CD4+CD25+ regulatory T cells. In addition, IL-10 may delay the rejection time of rabbit skin transplantation and prolong the survival time. Thus, the role of IL-10 in inhibited allograft rejection may be associated with CD4+CD25+ regulatory T cells and IL-10, and may be independent of TGF-β.
本研究旨在探讨重组人白细胞介素 -10（rhIL-10）或环孢素A（CsA）皮肤移植后移植皮肤的排斥反应和存活时间，以及外周血中调节性T细胞（Treg）、白细胞介素10（IL-10）和转化生长因子 -β（TGF-β）的变化，以及IL-10在免疫排斥机制中的作用。总共36只兔子被分为两组。将供体兔子的皮肤移植到一只受体兔子的背部。受体被随机分为六组，包括rhIL-10低剂量组（5μg/kg/d）、rhIL-10高剂量组（10μg/kg/d）、CsA低剂量组（5mg/kg/d）、CsA高剂量组（10mg/kg/d）、rhIL-10（5μg/kg/d）和CsA（5mg/kg/d）以及阴性对照生理盐水组（NS；1ml/d）。所有组在皮肤移植手术前一天开始进行为期十天的肌肉注射给药。移植后，在不同时间采集每只兔子的外周血。通过流式细胞术和酶联免疫吸附测定法测定CD4 + CD25 +调节性T细胞、IL-10和TGF-β的变化。与对照组相比，皮肤移植后实验组的排斥反应和存活时间更长。与两个CsA组和对照组相比，rhIL-10组的CD4 + CD25 +调节性T细胞比例在术后第4天和第7天显著上调。然而，TGF-β水平无显著差异。数据表明，IL-10浓度与CD4 + CD25 +调节性T细胞比例呈正相关。此外，IL-10可能延迟兔皮肤移植的排斥时间并延长存活时间。因此，IL-10在抑制同种异体移植排斥反应中的作用可能与CD4 + CD25 +调节性T细胞和IL-10有关，并且可能独立于TGF-β。
