转黄瓜花叶病毒反义 RNA 的植物对病毒的控制作用评估。

Anti-sense RNAs of cucumber mosaic virus in transgenic plants assessed for control of the virus.

机构信息

CSIRO Division of Horticulture, P.O. Box 350, 5001, Adelaide, S.A., Australia.

出版信息

Plant Mol Biol. 1988 Jul;11(4):463-71. doi: 10.1007/BF00039027.

Abstract

Three synthetic genes for the production of anti-sense RNA to different regions of the cucumber mosaic virus (CMV) genome were constructed using virus-derived double-stranded cDNA coupled to a promoter sequence from cauliflower mosaic virus. The genes were used to transform tobacco plants by a Ti plasmid vector. Transgenic plants obtained with the three constructs produced anti-sense RNA at different levels. Plants expressing each of the three anti-sense RNAs were inoculated with CMV and their sensitivity to the virus infection was compared with the non-transformed plants. Only one plant line which expressed relatively low levels of one of the anti-sense RNAs showed resistance to CMV but other plants expressing the same or the other two antisense RNAs had similar sensitivity to CMV infection as the non-transformed plants.

摘要

构建了三个用于生产黄瓜花叶病毒（CMV）基因组不同区域反义 RNA 的合成基因，该基因使用病毒衍生的双链 cDNA 与来自花椰菜花叶病毒的启动子序列连接。利用 Ti 质粒载体将基因转化为烟草植物。用三种构建体获得的转基因植物以不同水平表达反义 RNA。用 CMV 接种表达三种反义 RNA 中的每一种的植物，并将它们对病毒感染的敏感性与非转化植物进行比较。只有一条表达相对低水平的一种反义 RNA 的植物系表现出对 CMV 的抗性，但表达相同或其他两种反义 RNA 的其他植物对 CMV 感染的敏感性与非转化植物相似。