烟草花叶病毒RNA的核苷酸序列。
Nucleotide sequence of tobacco mosaic virus RNA.
作者信息
Goelet P, Lomonossoff G P, Butler P J, Akam M E, Gait M J, Karn J
出版信息
Proc Natl Acad Sci U S A. 1982 Oct;79(19):5818-22. doi: 10.1073/pnas.79.19.5818.
Abstract
Oligonucleotide primers have been used to generate a cDNA library covering the entire tobacco mosaic virus (TMV) RNA sequence. Analysis of these clones has enabled us to complete the viral RNA sequence and to study its variability within a viral population. The positive strand coding sequence starts 69 nucleotides from the 5' end with a reading frame for a protein of Mr 125,941 and terminates with UAG. Readthrough of this terminator would give rise to a protein of Mr 183,253. Overlapping the terminal five codons of this readthrough reading frame is a second reading frame coding for a protein of Mr 29,987. This gene terminates two nucleotides before the initiator codon of the coat protein gene. Potential signal sequences responsible for the capping and synthesis of the coat protein and Mr 29,987 protein mRNAs have been identified. Similar sequences within these reading frames may be used in the expression of sets of proteins that share COOH-terminal sequences.
摘要
寡核苷酸引物已被用于构建一个覆盖整个烟草花叶病毒（TMV）RNA序列的cDNA文库。对这些克隆的分析使我们能够完成病毒RNA序列，并研究其在病毒群体中的变异性。正义链编码序列从5'端起69个核苷酸处开始，具有一个编码Mr 125,941蛋白质的阅读框，并以UAG终止。该终止子的通读会产生一个Mr 183,253的蛋白质。与该通读阅读框的末端五个密码子重叠的是另一个编码Mr 29,987蛋白质的阅读框。该基因在衣壳蛋白基因起始密码子前两个核苷酸处终止。已鉴定出负责衣壳蛋白和Mr 29,987蛋白mRNA加帽和合成的潜在信号序列。这些阅读框内的相似序列可用于表达共享COOH末端序列的蛋白质组。
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本文引用的文献
1
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Nucleic Acids Res. 1980 Aug 25;8(16):3673-94. doi: 10.1093/nar/8.16.3673.
2
Two leaky termination codons in AMV RNA 1.禽成髓细胞瘤病毒RNA 1中的两个渗漏终止密码子。
FEBS Lett. 1980 Aug 25;118(1):67-71. doi: 10.1016/0014-5793(80)81220-8.
3
Assembly of tobacco mosaic virus: elongation towards the 3'-hydroxyl terminus of the RNA.烟草花叶病毒的组装：向RNA的3'-羟基末端延伸。
FEBS Lett. 1980 May 5;113(2):271-4. doi: 10.1016/0014-5793(80)80607-7.
4
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J Mol Biol. 1981 Nov 25;153(1):23-38. doi: 10.1016/0022-2836(81)90524-6.
5
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7
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