Gadani F, Mansky L M, Medici R, Miller W A, Hill J H
Research and Development, EniChem S.p.A., Milan, Italy.
Arch Virol. 1990;115(1-2):1-21. doi: 10.1007/BF01310619.
Historically, control of plant virus disease has involved numerous strategies which have often been combined to provide effective durable resistance in the field. In recent years, the dramatic advances obtained in plant molecular virology have enhanced our understanding of viral genome organizations and gene functions. Moreover, genetic engineering of plants for virus resistance has recently provided promising additional strategies for control of virus disease. At present, the most promising of these has been the expression of coat-protein coding sequences in plants transformed with a coat protein gene. Other potential methods include the expression of anti-sense viral transcripts in transgenic plants, the application of artificial anti-sense mediated gene regulation to viral systems, and the expression of viral satellite RNAs, RNAs with endoribonuclease activity, antiviral antibody genes, or human interferon genes in plants.
从历史上看，植物病毒病的防治涉及众多策略，这些策略常常相互结合，以便在田间提供有效的持久抗性。近年来，植物分子病毒学取得的巨大进展加深了我们对病毒基因组结构和基因功能的理解。此外，通过基因工程培育抗病毒植物最近为控制病毒病提供了有前景的额外策略。目前，其中最有前景的方法是在转 coat 蛋白基因的植物中表达 coat 蛋白编码序列。其他潜在方法包括在转基因植物中表达反义病毒转录本、将人工反义介导的基因调控应用于病毒系统，以及在植物中表达病毒卫星 RNA、具有核糖核酸内切酶活性的 RNA、抗病毒抗体基因或人类干扰素基因。
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