Zhao Wenhong, Yu Hui, Zhang Jianguo, Shu Li
Department of Preventive Medicine, Bengbu Medical College, Bengbu 233030, China.E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2013 Nov;33(11):1709-13.
To investigate the effect of different doses of glyphosate on apoptosis and expressions of androgen-binding protein (ABP) and vimentin mRNA in mouse Sertoli cells.
Primarily cultured mouse Sertoli cells incubated with different doses of glyphosate (60, 90, 120, 150 and 180 mg/L) for 24 h. The growth and morphological alterations in the cells were observed under inverted microscope, and the cell proliferation rate was evaluated withMTT assay. Hoechst 33342 staining was used to detect cell apoptosis after the treatment, and RT-PCR was performed to examine the changes in the expression of ABP and vimentin mRNAs.
Sertoli cells exposed to glyphosate showed a reduced cell volume, cell dissociation with occasional cell disruption. The proliferation of the exposed was suppressed with an increased rate of cell apoptosis and lowered expressions of ABP and vimentin mRNAs (P<0.05).
GLY can cause cellular damages, inhibit cell proliferation, induce cell apoptosis, and decrease expression of ABP and vimentin mRNAs in mouse Sertoli cells in vitro.
探讨不同剂量草甘膦对小鼠支持细胞凋亡及雄激素结合蛋白（ABP）和波形蛋白mRNA表达的影响。
将原代培养的小鼠支持细胞用不同剂量的草甘膦（60、90、120、150和180mg/L）处理24小时。在倒置显微镜下观察细胞的生长和形态变化，并用MTT法评估细胞增殖率。处理后用Hoechst 33342染色检测细胞凋亡，采用RT-PCR检测ABP和波形蛋白mRNA表达的变化。
暴露于草甘膦的支持细胞体积减小，细胞解离，偶尔出现细胞破裂。暴露组细胞增殖受到抑制，细胞凋亡率增加，ABP和波形蛋白mRNA表达降低（P<0.05）。
草甘膦可导致细胞损伤，抑制细胞增殖，诱导细胞凋亡，并降低体外培养的小鼠支持细胞中ABP和波形蛋白mRNA的表达。
