用毛细管电泳-三（2,2'-联吡啶）钌（II）电化学发光检测法分析奋乃静和氟奋乃静。

Analysis of perphenazine and fluphenazine by capillary electrophoresis coupled with tris (2,2'-bipyridyl) ruthenium (II) electrochemiluminescence detection.

机构信息

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.

出版信息

Talanta. 2014 Jan;118:1-6. doi: 10.1016/j.talanta.2013.09.048. Epub 2013 Oct 5.

DOI:10.1016/j.talanta.2013.09.048

PMID:24274263

Abstract

The coupling of end-column tris (2,2'-bipyridyl) ruthenium (II) electrochemiluminescence (ECL) detection with capillary electrophoresis (CE) was developed for the analysis of two antipsychotic drugs, perphenazine (PPH) and fluphenazine (FPH). The parameters related to CE separation and ECL detection, including the detection potential, the buffer pH value and concentration, the separation voltage, and Ru(bpy)₃(2+) concentration, were investigated in detail. Under optimum conditions, PPH and FPH were well separated and detected within 11 min. The linear ranges were 0.1-5 μM for PPH, and 0.1-7.5 μM for FPH, respectively. The limits of detection of PPH and FPH were 5 and 10 nM (S/N=3). The relative standard deviations (n=3) of the ECL intensity and the migration time were less than 2.5 and 0.65% in a day, and less than 3.4 and 1.7% in different three days. The proposed method was successfully applied to determine PPH and FPH in spiked urine samples with satisfactory results.

摘要

末端柱三（2,2'-联吡啶）钌（II）电化学发光（ECL）检测与毛细管电泳（CE）的偶联被开发用于分析两种抗精神病药物，奋乃静（PPH）和氟奋乃静（FPH）。详细研究了与 CE 分离和 ECL 检测相关的参数，包括检测电位、缓冲液 pH 值和浓度、分离电压和 Ru(bpy)₃(2+)浓度。在最佳条件下，PPH 和 FPH 在 11 分钟内得到很好的分离和检测。PPH 的线性范围为 0.1-5 μM，FPH 的线性范围为 0.1-7.5 μM。PPH 和 FPH 的检出限分别为 5 和 10 nM（S/N=3）。在一天内，ECL 强度和迁移时间的相对标准偏差（n=3）小于 2.5%和 0.65%，在不同的三天内小于 3.4%和 1.7%。该方法成功应用于测定加标尿样中的 PPH 和 FPH，结果令人满意。