卡介苗接种的健康受试者中牛分枝杆菌卡介苗诱导的人T细胞克隆：抗原特异性和淋巴因子产生
Mycobacterium bovis BCG-induced human T-cell clones from BCG-vaccinated healthy subjects: antigen specificity and lymphokine production.
作者信息
Mustafa A S, Kvalheim G, Degre M, Godal T
出版信息
Infect Immun. 1986 Sep;53(3):491-7. doi: 10.1128/iai.53.3.491-497.1986.
Abstract
A total of 121 human T-cell clones were raised from nine Mycobacterium bovis BCG-vaccinated healthy individuals. Three clones were autoreactive, 74 responded to BCG in the presence of antigen-presenting cells, and the others required in addition exogenous interleukin 2. Only one clone was CD8+ CD4-, and the rest were CD4+ CD8-. Testing with a panel of mycobacteria suggested that the clones were recognizing epitopes of varied specificity. Out of 44 clones tested, 15 were specific to BCG and Mycobacterium tuberculosis, 22 showed limited cross-reactivity, and 8 were broadly cross-reactive. None of the 22 BCG responder clones could differentiate between Danish, French, Prague, and Moreau strains of BCG. BCG and M. tuberculosis H37Rv also paralleled very closely; however, 6 of 18 BCG- and M. tuberculosis H37Rv-responding clones did not proliferate to Mycobacterium africanum. BCG- and M. tuberculosis H37Rv-specific as well as cross-reactive T-cell clones could be induced to produce interleukin 2, gamma interferon, and granulocyte-macrophage colony-stimulating factor activity.
摘要
从9名接种卡介苗的健康个体中总共培养出121个人类T细胞克隆。其中3个克隆具有自身反应性，74个在存在抗原呈递细胞的情况下对卡介苗有反应，其余的则还需要外源性白细胞介素2。只有1个克隆是CD8 + CD4 -，其余的都是CD4 + CD8 -。用一组分枝杆菌进行检测表明，这些克隆识别的表位具有不同的特异性。在检测的44个克隆中，15个对卡介苗和结核分枝杆菌具有特异性，22个表现出有限的交叉反应性，8个具有广泛的交叉反应性。22个对卡介苗有反应的克隆中没有一个能够区分丹麦、法国、布拉格和莫罗菌株的卡介苗。卡介苗和结核分枝杆菌H37Rv也非常相似；然而，在18个对卡介苗和结核分枝杆菌H37Rv有反应的克隆中，有6个对非洲分枝杆菌不增殖。对卡介苗和结核分枝杆菌H37Rv具有特异性以及交叉反应性的T细胞克隆可以被诱导产生白细胞介素2、γ干扰素和粒细胞 - 巨噬细胞集落刺激因子活性。
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