Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India.
Carbohydr Polym. 2014 Jan;99:264-9. doi: 10.1016/j.carbpol.2013.08.045. Epub 2013 Aug 28.
The sugarcane bagasse was biologically pretreated with three white-rot fungi; Pleurotus florida, Coriolopsis caperata RCK 2011 and Ganoderma sp. rckk-02, individually under solid-state fermentation. P. florida, C. caperata RCK 2011 and Ganoderma sp. rckk-02 degraded lignin up to 7.91, 5.48 and 5.58%, respectively. The lignocellulolytic enzymes produced by these fungi were also monitored during solid state fermentation of sugarcane bagasse. The fungal fermented sugarcane bagasse when hydrolyzed with crude cellulases from brown-rot fungus, Fomitopsis sp. RCK2010, released comparatively 1.5-2.4 fold higher sugars than in case of untreated sugarcane bagasse. The study demonstrated that white-rot fungal pretreatment improved the amenability of plant material for enzymatic hydrolysis.
甘蔗渣分别用三种白腐真菌(佛罗里达侧耳、卷毛革盖菌 RCK 2011 和灵芝 rckk-02)进行生物预处理,在固态发酵下进行。P. florida、C. caperata RCK 2011 和 Ganoderma sp. rckk-02 分别降解了 7.91%、5.48%和 5.58%的木质素。这些真菌在固态发酵甘蔗渣过程中产生的木质纤维素酶也得到了监测。当用褐腐菌 Fomitopsis sp. RCK2010 的粗纤维素酶水解真菌发酵的甘蔗渣时,与未处理的甘蔗渣相比,释放出的糖高出 1.5-2.4 倍。该研究表明,白腐真菌预处理提高了植物材料对酶水解的可及性。