Pandoraea sp. ISTKB 对甘蔗渣进行水下和固态预处理的比较：酶学和结构分析。
Comparison of submerged and solid state pretreatment of sugarcane bagasse by Pandoraea sp. ISTKB: Enzymatic and structural analysis.
机构信息
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India.
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India.
出版信息
Bioresour Technol. 2016 Mar;203:18-25. doi: 10.1016/j.biortech.2015.12.034. Epub 2015 Dec 18.
Pretreatment of sugarcane bagasse by Pandoraea sp. ISTKB was evaluated under submerged (SmF) and solid state (SSF) culture conditions. Number of bacteria was 2.7 times higher in SmF as compared with SSF. Enzymes produced under SmF were xylanase, LiP, MnP and laccase. In SSF xylanase and laccase were detected. CMCase, FPase and β-glucosidase were not detected. Delignification was highest in SmF with 19.94% and 10.43% removal of hemicelluloses and lignin, respectively. FTIR analysis suggested the degradation of lignin/hemicellulose component. SEM analysis showed pores were three times bigger in SmF as compared with raw bagasse. Maximum CR dye was absorbed by treated SmF bagasse. Enzymatic saccharification increased by 3.7 times after SmF treatment in comparison to raw bagasse. Pretreatment of bagasse by Pandoraea sp. ISTKB was more efficient under SmF than SSF. High negative correlation between saccharification vs lignin/hemicelluloses content justified the need for pretreatment of lignocellulosic waste before saccharification.
对 Pandoraea sp. ISTKB 在液体深层发酵（SmF）和固态发酵（SSF）条件下对甘蔗渣的预处理进行了评估。与 SSF 相比，SmF 中的细菌数量高 2.7 倍。SmF 下产生的酶有木聚糖酶、LiP、MnP 和漆酶。在 SSF 中检测到木聚糖酶和漆酶。未检测到CMC 酶、FP 酶和β-葡萄糖苷酶。SmF 中的木质素脱除率最高，分别为 19.94%和 10.43%，半纤维素和木质素的去除率分别为 19.94%和 10.43%。FTIR 分析表明木质素/半纤维素成分发生了降解。SEM 分析表明，SmF 处理后的孔比原蔗渣大三倍。经处理的 SmF 蔗渣对 CR 染料的吸附量最大。与原蔗渣相比，SmF 处理后酶解增加了 3.7 倍。与 SSF 相比，Pandoraea sp. ISTKB 在 SmF 下对蔗渣的预处理更有效。糖化率与木质素/半纤维素含量之间的高度负相关表明，在糖化前需要对木质纤维素废物进行预处理。
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